番茄早疫病菌啶菌噁唑抗性菌株的适合度  

Fitness of Pyrisoxazole-resistant Isolate Inciting Tomato Early Blight

作　　者：史晓晶 王华杰 石瑞 赵丽娟 SHI Xiaojing;WANG Huajie;SHI Rui;ZHAO Lijuan(Department of Biology,Xinzhou Teachers University,Xinzhou 034000,China)

机构地区：[1]忻州师范学院生物系,山西忻州034000

出　　处：《云南农业大学学报（自然科学版）》2022年第4期553-558,共6页Journal of Yunnan Agricultural University:Natural Science

基　　金：山西省基础研究计划(20210302124623);山西省高等学校科技创新项目(2021L464);忻州市科技计划应用基础项目(20210104)。

摘　　要：【目的】评估番茄早疫病菌——茄链格孢菌(Alternaria solani)对啶菌噁唑的抗性风险,探讨抗性菌株的适合度变化。【方法】采用菌丝生长速率法测定抗性菌株的啶菌噁唑敏感性、抗性遗传稳定性及生长速率,评估抗性菌株的产孢量、孢子萌发率、孢子竞争力、致病性与产毒量的变化。【结果】抗性菌株对啶菌噁唑处于低至中抗水平,且在继代培养20代后抗性仍可稳定遗传,但菌株对啶菌噁唑的抗性越强,生长速率越慢;抗性菌株的产孢量增多,孢子萌发率未发生变化,但孢子竞争力变差;其产毒量下降,致病性降低,在啶菌噁唑压力下致病性强于敏感菌株。【结论】当番茄早疫病菌对啶菌噁唑产生抗性后,抗性可稳定遗传,繁殖能力增强,生存能力和寄生能力减弱,但可借助啶菌噁唑对敏感群体的压制而壮大抗性菌群。需加强田间抗性监测,并制订啶菌噁唑抗性发展延缓策略。[Purpose]To evaluate the resistance risk of Alternaria solani inciting tomato early blight to pyrisoxazole,and the fitness of pyrisoxazole-resistant isolates was studied.[Methods]The sensitivity to pyrisoxazole,stability of resistance,and growth rate of resistant isolates were analyzed by mycelial growth rate method.And the sporulation,germination rate,spore competitiveness,pathogenicity,and toxin production were also assessed.[Results]Resistant isolates were low to moderate resistant to pyrisoxazole,and the resistance could be inherited steadily after 20 generations of subculture.The mycelial growth of resistant isolates declined along with reduction of sensitivity.Their sporulation increased and spore competitiveness became weaker,but germination rate did not vary.Their toxin production declined so that the pathogenicity turned weaker.However,the pathogenicity of resistant isolates was stronger than that of sensitive one under the impact of pyrisoxazole.[Conclusion]The pyrisoxazole-resistant isolates,whose resistance can be inherited steadily,pos-sessed enhanced reproductive capacity,weaken viability and parasitic ability,but they can be expand their populations by the suppression of sensitive populations with pyrisoxazole.It is necessary to monitor the field resistance continually,and make the prevented strategies for development of pyrisoxazole resistance.

关 键 词：番茄早疫病 啶菌噁唑 茄链格孢菌 适合度 

分 类 号：S436.412.14[农业科学—农业昆虫与害虫防治]