牛科物种FEZF2基因分子特征、功能预测及进化分析  

Molecular Characterization,Functional Prediction and Evolution-ary Analysis of FEZF2 Gene in Species of Bovidae

作　　者：保志鹏[1] 涂兴调 张自芳[1] 谷丽娇 范新阳[2] 钱林东[1] 苗永旺[2] BAO Zhipeng;TU Xingtiao;ZHANG Zifang;GU Lijiao;FAN Xinyang;QIAN Lindong;MIAO Yongwang(Department of Animal Husbandry&Veterinary Medicine,Yunnan Agricultural College of Vocational Education,Kunming 650212,China;Faculty of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业职业技术学院畜牧兽医学院,云南昆明650212 [2]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2022年第4期574-586,共13页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省教育厅科学研究基金项目(2018JS581);国家自然科学基金项目(31760659)。

摘　　要：【目的】揭示牛科物种FEZF2基因的功能及其差异。【方法】从NCBI和Ensembl数据库下载牛科主要家畜FEZF2基因序列及用于比较的非牛科物种的同源序列,采用生物信息学和比较基因组学方法对牛科家畜FEZF2基因的结构、编码蛋白的理化特征、氨基酸组成、基序和保守结构域、高级结构、参与的生物学路径、分子功能及进化关系进行比较分析。【结果】牛科动物间FEZF2基因转录区的结构存在差异,表现为非翻译区和内含子的序列长度不一致,但它们的编码序列(coding sequence,CDS)结构模式一致,与非牛科动物的CDS结构具有共线性关系。牛科动物FEZF2蛋白都是在核内发挥生物学功能的亲水性蛋白,无信号肽序列及跨膜结构域,都含有1个COG5048保守的锌指结构域。预测显示:FEZF2蛋白参与的生物学过程主要与神经元发育调节有关,分子功能主要是与染色质结合、转录激活或抑制有关。牛科与非牛科哺乳动物的FEZF2序列都存在1个连续的甘氨酸序列重复区,水牛、牦牛、绵羊和山羊均为13G型,但普通牛和瘤牛存在12G和13G两个等位基因。各牛科物种FEZF2蛋白在氨基酸组成、序列一致性、理化特征、基序、结构域和高级结构上存在相似性,但在牛科的不同属间存在一定差异。系统发育分析显示:牛科同属动物的FEZF2蛋白遗传关系较近。【结论】本研究揭示牛科家畜间FEZF2基因CDS的结构模式高度一致;FEZF2作为核内功能蛋白可能参与了牛科物种的神经元发育和机体免疫等过程的基因表达调控。[Purpose]To reveal the functions and differences of FEZF2 gene in species of Bovidae family.[Methods]The sequences of FEZF2 gene in domestic animals of Bovidae and homologous sequences of non-Bovidae species for comparison were downloaded from NCBI and Ensembl databases,the structure of FEZF2 gene,and the physicochemical characteristics,amino acid composition,motifs and conserved domains,advanced structures,biological pathways involved,molecular functions and evolutionary relationships of its encoded proteins were compared and analyzed using the methods of bioinformatics and comparative genomics.[Results]The structure of the transcription region of FEZF2 gene is different among species of Bovidae,which mainly shows that the sequence length of the untranslated regions and introns is inconsistent.However,the structural pattern of their coding sequence(CDS)is consistent,which displays collinearity with the CDS structure of non-Bovidae animals.FEZF2 protein of Bovidae species is a hydrophilic protein without a signal peptide sequence and a transmembrane domain that performs biological functions in the nucleus,and contains a conserved zinc finger domain of COG5048.The prediction showed that the biological process involved in this protein is mainly related to the regulation of neuronal development,and the molecular function is mainly related to chromatin binding,transcriptional activation or inhibition.There is a continuous glycine repeat region in FEZF2 sequences of Bovidae and non-Bovidae mammals.Buffalo,yak,sheep and goat are all 13G type,but cattle and zebu have two alleles of 12G and 13G.The amino acid composition,sequence consistency,physicochemical characteristics,motifs,domains and advanced structures of FEZF2 protein among the species of Bovidae are similar,but there are some differences among different genera of Bovidae.Phylogenetic analysis showed that the FEZF2 proteins of the same genus of Bovidae are closely related to each other.[Conclusion]This study reveals that the structural pattern of FEZF2 gene CDS

关 键 词：牛科物种 FEZF2基因 分子特征 基因功能 生物信息学与比较基因组学分析 

分 类 号：S823.2[农业科学—畜牧学]