一株深色有隔内生真菌的Cd吸附特征研究  

Study on Cadmium Adsorption Characteristics of a Dark Septate Endophytes

作　　者：杨仁媛 梁新然 李祖然 湛方栋[1] 李涛[3] 赵之伟[3] YANG Renyuan;LIANG Xinran;LI Zuran;ZHAN Fangdong;LI Tao;ZHAO Zhiwei(College of Resources and Environment,Yunnan Agricultural University,Kunming 650201,China;College of Horticulture and Landscape,Yunnan Agricultural University,Kunming 650201,China;State Key Laboratory of Biological Resources Protection and Utilization of Yunnan Province,Yunnan University,Kunming 650091,China)

机构地区：[1]云南农业大学资源与环境学院,云南昆明650201 [2]云南农业大学园林园艺学院,云南昆明650201 [3]云南大学省部共建云南生物资源保护与利用国家重点实验室,云南昆明650091

出　　处：《云南农业大学学报（自然科学版）》2022年第4期684-691,共8页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然科学基金项目(41877130);云南省中青年学术和技术带头人后备人才培养项目(2018HB043)。

摘　　要：【目的】研究镉(Cd)对深色有隔内生真菌(dark septate endophytes,DSE)的吸附特征,为利用DSE吸附去除Cd污染提供理论依据。【方法】采用1株DSE——嗜鱼外瓶霉(Exophiala pisciphila ACCC32496)作为研究材料,探讨菌丝吸附Cd的影响因子(pH值、初始Cd质量浓度和吸附时间)及吸附特性。【结果】嗜鱼外瓶霉吸附Cd的最佳pH值为5,并且随着初始Cd质量浓度的增加,吸附量增加,直至达到饱和状态,最大吸附量为14.14 mg/g。吸附过程分为快速反应和慢速反应阶段,60 min内Cd吸附量为11.37 mg/g,吸附过程符合Langmuir吸附等温模型,吸附动力学符合准二级速率方程。红外光谱分析表明:嗜鱼外瓶霉吸附Cd涉及氨基、酰胺基、羧基、羟基和磷酰基等基团。解吸试验表明:HNO3和Na2EDTA从嗜鱼外瓶霉菌丝上解吸Cd的能力最强。【结论】DSE嗜鱼外瓶霉(E.pisciphila ACCC32496)对Cd具有较好的吸附效果,主要依靠细胞壁上的氨基、酰胺基、羧基、羟基和磷酰基等基团吸附去除Cd。[Purpose]To study the adsorption characteristics of cadmium(Cd)for dark septate endophytes(DSE),providing theoretical basis for using DSE adsorption to remove Cd pollution.[Method]Using a DSE(Exophiala pisciphila ACCC32496)as a research material to explore the influence factor(pH value,initial Cd mass concentration and adsorption time)of the mycelium adsorption Cd.[Results]The optimal pH value for Cd adsorption by E.pisciphila was 5,and as the initial Cd mass concentration increased,the adsorption amount increased until it reached the saturation state,and the maximum adsorption amount was 14.14 mg/g.The adsorption process was divided into a fast reaction and a slow reaction stage.The Cd adsorption amount in 60 min was 11.37 mg/g.The adsorption process conformed to Langmuir adsorption isothermal model,and adsorption dynam-ics conformed to the quasi-secondary rate equation.Infrared spectrum analysis showed that E.pis-ciphila adsorption Cd involved amino,amido,carboxyl,hydroxyl,and phosphoryl groups.The ana-lysis test showed that HNO3 and Na2EDTA had the strongest ability to desorb Cd from the E.pis-ciphila.[Conclusions]DSE E.pisciphila ACCC32496 has a good adsorption effect on Cd,mainly relying on the amino,amido,carboxyl,hydroxyl,and phosphoryl groups on the cell wall.

关 键 词：深色有隔内生真菌 镉 嗜鱼外瓶霉 基团 吸附 

分 类 号：S432.44[农业科学—植物病理学]