持续性次氯酸消毒对内镜终末漂洗水的消毒效果  

Disinfection effect of persistent hypochlorous acid on final rinsing water for endoscope

作　　者：王萍 韩梦鸽 沈国锋[3] 李娜 张鑫 潘玥玮 史庆丰 高晓东 孙伟 WANG Ping;HAN Meng-ge;SHEN Guo-feng;LI Na;ZHANG Xin;PAN Yue-wei;SHI Qing-feng;GAO Xiao-dong;SUN Wei(Endoscopy Center, Zhongshan Hospital, Fudan University, Shanghai 200032, China;Department of Infection Management, Zhongshan Hospital, Fudan University, Shanghai 200032, China;Department of Infection Ma-nagement, Minhang Hospital, Fudan University, Shanghai 201199, China;Department of Infectious Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China)

机构地区：[1]复旦大学附属中山医院内镜中心,上海200032 [2]复旦大学附属中山医院感染管理科,上海200032 [3]复旦大学附属闵行医院感染管理科,上海201199 [4]复旦大学附属中山医院感染病科,上海200032

出　　处：《中国感染控制杂志》2022年第7期617-622,共6页Chinese Journal of Infection Control

基　　金：上海市科研计划项目(19DZ2280100)。

摘　　要：目的探讨持续性次氯酸灌注对内镜终末漂洗水的消毒效果。方法基线采集内镜终末漂洗水的水样,进行微生物培养和宏基因组二代测序鉴定。引入微酸性次氯酸水发生机后,在安装后的特定时间收集水样进行培养和宏基因组二代测序分析。结果安装前共采集水样27份,其中10份合格,合格率为37.03%。不合格水样可检出放线根瘤菌、嗜麦芽窄食单胞菌、黄色微杆菌、贪铜菌、藤泽甲基杆菌、少动鞘氨醇单胞菌等水源性细菌;安装后共采集水样54份,均为合格。宏基因组二代测序分析显示,安装前的样本中可检测出大量皮氏罗尔斯顿菌、耐金属贪铜菌、奥斯陆莫拉菌、日本慢生根瘤菌、水生甲基杆菌、戈登分枝杆菌、新鞘氨醇杆菌等细菌序列,消毒后主要为宏基因测序分析常见的背景细菌。结论持续性次氯酸灌注消毒可有效清除水管路中的细菌,并降低水管路中生物膜的形成,提升内镜终末漂洗水的质量。Objective To discuss the disinfection effect of persistent hypochlorous acid supply to final rinse water for endoscope.Methods Water specimens of final rinse water for endoscope were collected at baseline,and identified by microbial culture and metagenomic next-generation sequencing(mNGS).After introducing a slightly acidic hypochlorous acid water generator,water specimens were collected at a specific time after installation for culture and mNGS analysis.Results A total of 27 water specimens were collected before installation,and 10(37.03%)were qualified.Aquatic bacteria such as Rhizobiumradiobacter,Stenotrophomonas maltophilia,Microbacterium flavum,Cupriavidus,Methylobacterium fujisawaense and Sphingomonas paucimobilis can be detected in unqualified water specimens,54 water specimens were collected after installation,and all of them were qualified.mNGS shows that abundant sequences of Ralstonia Pichia,Cupriavidus metallidurans,Moraxella osloensis,Rhizobium japonicum,Aquatic methylbacterium,Mycobacterium Gordon and Sphingosine neobacterium could be detected in water specimens before installation,but only background bacteria in mNGS were found after disinfection.Conclusion Persistent hypochlorous acid supply can effectively remove bacteria and reduce biofilm formation in water pipeline,improving the quality of final rinse water for endoscope.

关 键 词：次氯酸 持续性消毒 内镜消毒 宏基因组二代测序 水源性细菌 

分 类 号：R187.2[医药卫生—流行病学]