泛耐药鲍曼不动杆菌生物被膜相关基因及整合子与耐药性的关系  

Relationship between extensively drug-resistant Acinetobacter baumannii biofilm-related genes,integrons and drug resistance

作　　者：蔡杨 凌保东[1,2] CAI Yang;LING Bao-dong(Key Laboratory of Sichuan Province College for Structu-ral Specific Small Molecule Drug Research,Chengdu 610500,China;School of Pharmacy,Chengdu Medical College,Chengdu 610500,China)

机构地区：[1]成都医学院结构特异性小分子药物研究四川省高校重点实验室,四川成都610500 [2]成都医学院药学院,四川成都610500

出　　处：《中国感染控制杂志》2022年第7期690-696,共7页Chinese Journal of Infection Control

基　　金：国家自然科学基金面上项目(81373454)。

摘　　要：目的了解临床分离鲍曼不动杆菌(AB)的耐药状况,以及生物被膜相关基因和整合子的分布情况,探讨泛耐药鲍曼不动杆菌(XDR-AB)生物被膜相关基因及整合子与耐药性的关系。方法2019—2020年成都某医院临床标本分离的59株AB,采用微量肉汤法检测17种抗菌药物对AB的最低抑菌浓度(MIC),聚合酶链反应(PCR)法扩增17个生物被膜相关基因及Ⅰ、Ⅱ、Ⅲ类整合酶基因,同时对Ⅰ类整合酶阳性菌株可变区进行扩增和测序。结果59株AB对米诺环素耐药率为1.69%,对亚胺培南、美罗培南耐药率分别为67.80%、71.19%,对其他抗菌药物的耐药率为59.32%~84.75%。59株AB中XDR-AB 40株(67.80%),多重耐药菌株(MDR-AB)5株(8.47%),敏感菌株14株(23.73%)。59株AB 17种生物被膜相关基因中,6种生物被膜相关基因bfmR、bfmS、csuC、csuD、csuE、pgaD检出率为100%,其他11种生物被膜相关基因检出率为74.58%~98.31%。XDR-AB 4种生物被膜相关基因abaI、epsA、pglC、ompA检出率分别为100%、95.00%、87.50%、100%,高于敏感AB的64.29%、7.14%、42.86%、50.00%(均P<0.05)。intI-1整合酶基因检出率:AB为66.10%(39/59),XDR-AB为90.00%(36/40),敏感AB菌株未检出。39株Ⅰ类整合酶阳性菌株38株检测到Ⅰ类整合子可变区,其中XDR-AB 35株,MDR-AB 3株。结论AB中XDR-AB检出率高,推测XDR-AB携带的生物被膜相关基因abaI、epsA、pglC、ompA及Ⅰ类整合子与耐药性密切相关。Objective To investigate the drug resistance of clinically isolated Acinetobacter baumannii(AB)and distribution of biofilm-related genes and integrons,explore the relationship between extensively drug-resistant AB(XDR-AB)biofilm-related genes,integrons and drug resistance.Methods Minimum inhibitory concentration(MIC)of 17 kinds of antimicrobial agents against 59 AB strains isolated from clinical specimens of a hospital in Chengdu from 2019 to 2020 were performed by micro-broth method,17 biofilm-related genes and classⅠ,ⅡandⅢintegrase genes were amplified by polymerase chain reaction(PCR),variable regions of class I integrase positive strains were amplified and sequenced.Results Resistance rate of 59 AB strains to minocycline was 1.69%,to imipenem and meropenem were 67.80%and 71.19%respectively,and to other antimicrobial agents were 59.32%-84.75%.Among 59 AB strains,40 strains(67.80%)were XDR-AB,5 strains(8.47%)were multidrug-resis-tance AB(MDR-AB),and 14 strains(23.73%)were sensitive strains.Among 17 biofilm-related genes of 59 AB strains,detection rates of 6 biofilm-related genes bfmR,bfmS,csuC,csuD,csuE,and pgaD were 100%,detection rates of the other 11 biofilm-related genes were 74.58%-98.31%.Detection rates of 4 biofilm-related genes abaI,epsA,pglC,and ompA in XDR-AB were 100%,95.00%,87.50%and 100%respectively,which were higher than those in sensitive AB strains(64.29%,7.14%,42.86%and 50.00%,all P<0.05).The detection rate of intI-1 integrase gene of AB and XDR-AB were 66.10%(39/59)and 90.00%(36/40)respectively,and sensitive AB strain was not found.Among 39 class I integrase positive strains,38 strains of class I integron variable region were detected,including 35 XDR-AB strains and 3 MDR-AB strains.Conclusion Detection rate of XDR-AB among AB is high,it is speculated that the biofilm-related genes abaI,epsA,pglC,ompA and class I integron carried by XDR-AB are closely related to drug resistance.

关 键 词：泛耐药鲍曼不动杆菌 鲍曼不动杆菌 生物被膜 整合子 耐药性 

分 类 号：R181.32[医药卫生—流行病学]