不同梅毒血清学检测策略应用于梅毒诊断中的价值对比  

Comparison of the Value of Different Syphilis Serologic Testing Strategies Applied in the Diagnosis of Syphilis

作　　者：陈俊生 张旭明 CHEN Junsheng;ZHANG Xuming(Department of Laboratory Medicine,Shiyan People’s Hospital of Baoan District,Shenzhen,Guangdong,518108)

机构地区：[1]深圳市宝安区石岩人民医院检验科,广东深圳518108

出　　处：《智慧健康》2022年第17期9-11,15,共4页Smart Healthcare

摘　　要：目的探讨不同梅毒血清学检测策略应用于梅毒诊断中的价值。方法本研究所选取的是深圳市宝安区石岩人民医院2019年11月-2020年11月收治的80例梅毒患者作为观察组,并且选取同时期体检的50例非梅毒患者作为对照组。所纳入的全部患者均需要收集血液标本,通过应用酶联免疫吸附试验(TP-ELISA)、梅毒螺旋体明胶凝集试验(TPPA)及甲苯胺红不加热血清试验(TRUST)3种检测方法,以临床病理诊断为金标准,比较三种不同的检测方法对梅毒诊断阳性检测率以及诊断价值(确诊率、误诊率以及敏感性、特异性、阳性预测值、阴性预测值)的不同。结果TP-ELISA和TPPA法诊断阳性率均高于TRUST,差异具有统计学意义(χ2=17.644,P<0.05),但两者之间比较差异无统计学意义(χ2=1.344,P>0.05);TP-ELISA的敏感性及阴性预测值高于TRUST,TPPA的特异性及阳性预测值高于TP-ELISA及TRUST,差异具有统计学意义(P<0.05)。结论相较于TRUST,TP-ELISA和TPPA法可提高对梅毒患者的检测诊断。Objective To investigate the value of different syphilis serological testing strategies applied in the diagnosis of syphilis.Methods The study selected 80 cases of syphilis patients admitted to our hospital from November 2019 to November 2020 as the observation group,and 50 cases of non syphilis patients with simultaneous physical examination were selected as the control group.Blood samples were collected from all included patients,and three assays,the Treponema pallidum gelatin agglutination test(TPPA)and toluidine red unheated serum test(TRUST),were used to compare the positive test rates as well as the diagnostic values of the three different assays for diagnosis of syphilis,using clinicopathological diagnosis as the gold standard(confirmation rate misdiagnosis rate as well as varying sensitivity,specificity,positive predictive value,negative predictive value).Results The diagnostic positivity of TP-ELISA and TPPA methods were higher than TRUST with statistical significance(χ2=17.644,P<0.05),but there was no significant difference between them(χ2=1.344,P>0.05);the sensitivity and negative predictive value of TP-ELISA were higher than TRUST,and the specificity and positive predictive value of TPPA were higher than TP-ELISA and TRUST,with statistically significant differences(P<0.05).Conclusion The TP-ELISA and TPPA assays improve the test diagnosis for patients with syphilis relative to TRUST.

关 键 词：梅毒 血清学检测 酶联免疫吸附试验 梅毒螺旋体明胶凝集试验 甲苯胺红不加热血清试验 

分 类 号：R759.1[医药卫生—皮肤病学与性病学] R446.6[医药卫生—临床医学]