7种新发传染病高通量悬浮芯片检测方法的建立  

Establishment of a Multiplex Liquid Bead Array for Seven Types of Emerging Infectious Diseases

作　　者：闫冀焕[1] 沈军[1] 周新霞 周汝明[2] 梁宏 Yan Jihuan;Shen Jun;Zhou Xinxia;Zhou Ruming;Liang Hong(Hebei International Travel Health Care Center(Shijiazhuang Customs Port Clinic),Shijiazhuang Hebei 050091;Hebei Provincial People’s Hospital,Shijiazhuang Hebei 050000;Suifenhe Customs Integrated Technology Centre(Suifenhe Customs Port Clinic),Suifenhe Heilongjiang 157399)

机构地区：[1]河北国际旅行卫生保健中心(石家庄海关口岸门诊部),河北石家庄050091 [2]河北省人民医院,河北石家庄050000 [3]绥芬河海关综合技术中心(绥芬河海关口岸门诊部),黑龙江绥芬河157399

出　　处：《口岸卫生控制》2022年第1期39-43,54,共6页Port Health Control

基　　金：国家重点研发计划项目(2019YFF0302501);国家重点研发计划项目(2019YFF0302505);河北省重点研发计划项目(213777109D)。

摘　　要：目的建立7种新发传染病高通量悬浮芯片检测方法。方法根据现有DNA序列信息,设计并合成相关引物及探针,首先进行PCR扩增,产物与核酸探针微球组杂交后检测荧光信号值。结果建立的悬浮芯片方法检测ZIKA、LSA、CHIKV、WNV四种病原体的敏感性为10 DNA拷贝;MERS、H5N1、H7N9三种病原体的敏感性为100 DNA拷贝。结论建立了可同时检测7种新发传染病检测方法,为新发传染病有效防控提供了支持。Objective To develop a method for seven types of emerging infectious diseases.Methods According to the available DNA sequence information,the related primers and probes were designed and synthesized.The PCR products were amplified and the fluorescence signals were detected after hybridized with nucleic acid probe microsphere.Results The susceptibility of Zika,LSA,CHIKV and WNV were 10 DNA copy,and the susceptibility of MERS,H5N1 and H7N9 were 100 DNA copy.Conclusion A detection method for seven types of emerging infectious diseases has been developed,and can support effective prevention of Emerging Infectious Diseases.

关 键 词：新发传染病 悬液芯片 病原体 检测方法 

分 类 号：R192.6[医药卫生—卫生事业管理]