虎杖提取物对小鼠肝癌原位移植瘤及miRNA-204-5p/Jarid2/PTEN信号通路的影响  

Effects of Polygonum Cuspidatum Extract on Orthotopic Transplanted Liver Cancer and miRNA-204-5p/Jarid2/PTEN Signaling Pathway in Mice

作　　者：李泽山 刘超[1] 申东方[1] 顾小晖 何艳新[1] LI Zeshan;LIU Chao;SHEN Dongfang;GU Xiaohui;HE Yanxin(The Second Affiliated Hospital of Zhengzhou University,Zhengzhou,Henan 450000)

机构地区：[1]郑州大学第二附属医院,河南郑州450000

出　　处：《智慧健康》2022年第10期179-182,192,共5页Smart Healthcare

摘　　要：目的观察虎杖提取物对小鼠肝癌原位移植瘤及miRNA-204-5p/Jarid2/PTEN信号通路的影响。方法将32只BALB/c小鼠肝癌细胞原位移植瘤模型制备成功,随机分为模型组、虎杖提取物低、中、高剂量组,每组8只,另取8只健康小鼠作为对照组,虎杖提取物低、中、高剂量组分别给予虎杖提取物0.1mL、0.2mL及0.4mL配于适量体积的生理盐水中并灌胃,模型组及对照组给予低、中、高剂量组相同体积生理盐水灌胃,均连续处理28 d。观察各组小鼠存活率、体重、器官指数(包括肝、脾及胸腺)、平均瘤重及抑瘤率;ELISA法检测各组小鼠外血清肝功能指标丙氨酸氨基转移酶(ALT),天门冬氨酸氨基转移酶(AST),谷氨酰转移酶(GGT)的变化;Real-time PCR检测各组小鼠肿瘤组织中miRNA-204-5p表达,Western blot检测各组小鼠肿瘤组织中Jarid2、PTEN蛋白表达。结果各组小鼠存活率无明显差异(P>0.05);与对照组比较,模型组体重及胸腺指数降低(P<0.05),肝脏指数、脾指数、ALT、AST及GGT含量升高(P<0.05);与模型组比较,虎杖提取物低、中、高剂量组体重、胸腺指数、抑瘤率及肿瘤组织中miRNA-204-5p、PTEN表达均升高(P<0.05),肝脏指数、脾指数、瘤重、ALT、AST、GGT含量及肿瘤组织中Jarid2表达均降低(P<0.05),且存在剂量依赖性(P<0.05)。结论虎杖提取物可有效抑制小鼠原位移植瘤生长,改善内脏指数,其机制可能与影响miRNA-204-5p/Jarid2/PTEN信号通路有关。Objective To observe the effects of Polygonum cuspidatum extract on orthotopic transplanted liver cancer and miRNA-204-5p/Jarid2/PTEN signal pathway in mice.Methods Thirtytwo BALB/c mice with orthotopic transplantation of liver cancer cells were randomly divided into model group,low,medium and high dose groups of Polygonum cuspidatum extract,with 8 mice in each group,and 8 healthy mice as the control group.The low,medium and high dose groups of Polygonum cuspidatum extract were gavaged with 0.1 mL,0.2 mL and 0.4 mL of Polygonum cuspidatum extract respectively.The model group and the control group were gavaged with the same volume of normal saline in the low,medium and high dose groups for 28 days.The survival rate,body weight,organ index(including liver,spleen and thymus),average tumor weight and tumor inhibition rate of mice in each group were observed;The changes of alanine aminotransferase(ALT),aspartate aminotransferase(AST)and glutamyltransferase(GGT)were detected by ELISA;Real time PCR was used to detect the expression of miRNA-204-5p in tumor tissues of mice in each group,and Western blot was used to detect the expression of Jarid2 and PTEN protei其n in tumor tissues of mice in each group.Results There was no significant difference in the survival rate of mice in each group(P>0.05);Compared with the control group,the body weight and thymus index of the model group decreased(P<0.05),and the contents of liver index,spleen index,ALT、AST and GGT increased(P<0.05);Compared with the model group,the body weight,thymus index,tumor inhibition rate and the expression of miRNA-204-5p and PTEN in tumor tissues increased in low,medium and high dose groups of Polygonum cuspidatum extract(P<0.05),while the contents of liver index,spleen index,tumor weight,ALT、AST and GGT and the expression of Jarid2 in tumor tissues decreased in a dose-dependent manner(P<0.05).Conclusion Polygonum cuspidatum extract can effectively inhibit the growth of orthotopic transplanted tumor and improve visceral index in mice.Its mechanism m

关 键 词：虎杖提取物 肝癌 微小RNA 含有JmjC域SMCX蛋白 人第10号染色体缺失的磷酸酶 

分 类 号：R285.5[医药卫生—中药学]