不同激素对山韭种子萌发特性的影响  

Effects of Different Hormones on Seed Germination Characteristics of Allium senescens L.

作　　者：李韦瑶 肖汇川 张碧莹 李杰[1] 苏滢淼 崔国文[1] 秦立刚[1] LI Weiyao;XIAO Huichuan;ZHANG Biying;LI Jie;SU Yingmiao;CUI Guowen;QIN Ligang(College of Animal Science,Northeast Agricultural University,Harbin Heilongjiang 150030,China;College of Food Science,Northeast Agricultural University,Harbin Heilongjiang 150030,China)

机构地区：[1]东北农业大学动物科学技术学院,黑龙江哈尔滨150030 [2]东北农业大学食品学院,黑龙江哈尔滨150030

出　　处：《种子》2022年第6期76-83,共8页Seed

基　　金：国家自然科学基金(31702160);哈尔滨市科技创新人才项目(2017 RAQXJ 007)。

摘　　要：试验采用吲哚乙酸(Indole-3-acetic acid,IAA)、乙烯利(Ethrel,ETH)和萘乙酸(Naphthalene acetic acid,NAA)对山韭种子进行浸种处理,以蒸馏水为对照,测定各处理条件下的种子发芽指标和形态指标,进而选择可明显提升种子发芽指标的激素浓度,测定该处理下的种子生理指标。结果表明,与ck相比,100 mg/L IAA和50 mg/L ETH对山韭种子的萌发和形态有显著促进作用,而NAA处理抑制种子萌发和生长。100 mg/L IAA处理显著提高山韭种子可溶性蛋白含量和蛋白酶活性。50 mg/L ETH处理显著增加了可溶性蛋白、可溶性糖含量、脂肪酶活性、内源IAA和ETH含量。200 mg/L NAA处理对可溶性蛋白、可溶性糖含量、蛋白酶活性和内源ETH含量有显著提升。主成分分析和相关性分析表明,山韭种子萌发特性各指标之间均呈显著正相关,与内源IAA含量呈显著负相关。The seeds were soaked with indole acetic acid,ethephon and naphthalene acetic acid,and distilled water was used as the control to determine the seed germination indexes and morphological indexes under each treatment condition.The hormone concentration which could significantly improve seed germination index was selected to determine the seed physiological index under the treatment.The results showed that compared with the control,100 mg/L IAA and 50 mg/L ETH could significantly promote the germination and morphological properties of Allium senescens seeds,while NAA treatment inhibited the seed germination and growth.Treatment with 100 mg/L IAA significantly increased the soluble protein content and protease activity of Chinese chives.Treatment with 50 mg/L ETH significantly increased soluble protein,soluble sugar content,lipase activity,IAA and ETH content.Treatment with 200 mg/L NAA also significantly increased soluble protein,soluble sugar content,protease activity and ETH content.Principal component analysis and correlation analysis showed that there was a significant positive correlation between the various indexes of the germination characteristics of Allium senescens,and a significant negative correlation with the content of endogenous IAA.

关 键 词：山韭 种子萌发 激素处理 生理生化 

分 类 号：S647[农业科学—蔬菜学]