基于生物信息学方法的沙眼衣原体主要外膜蛋白免疫优势表位预测及分析  

Prediction and analysis of immunodominant epitope of Chlamydia trachomatis MOMP based on bioinformatics

作　　者：李明洋 徐远久 鲁小龙 张雷 苏惠婷 朱珊丽[2] 张友秀 LI Mingyang;XU Yuanjiu;LU Xiaolong;ZHANG Lei;SU Huiting;ZHU Shanli;ZHANG Youxiu(Department of Laboratory,West China-Guang'an Hospital,Sichuan University,Guang'an 638000,China;不详)

机构地区：[1]四川大学华西广安医院检验科,四川广安638000 [2]温州医科大学分子病毒与免疫研究所微生物学与免疫学教研室 [3]四川大学华西广安医院外围手术室

出　　处：《山东医药》2022年第20期24-29,共6页Shandong Medical Journal

基　　金：国家自然科学基金项目(31700160);温州市科技局资助项目(Y20170011)。

摘　　要：目的预测和分析沙眼衣原体主要外膜蛋白(MOMP)的免疫优势表位。方法以UniProt蛋白质数据库中检索CtMOMP的氨基酸序列为基础,针对MOMP的理化性质、二级结构、亲水性参数、表面可及性参数、极性参数、抗原性和柔韧性参数,预测其B细胞表位;用SYFPEITHI、IEDB及NetCTI 1.2 server预测MOMP的细胞毒性T淋巴细胞(CTL)表位;用RANKPEP及SYFPEITHI预测MOMP的辅助T细胞(Th)表位,筛选分值共同较高的多肽片段作为候选表位。应用GalaxyWEB对MOMP进行三维结构建模分析。结果MOMP由393个氨基酸残基组成,以无规则卷曲及α螺旋为主;推测MOMP的B细胞表位位于N端42~52 aa、161~179 aa、259~265 aa和349~358 aa区段;其CTL表位主要集中于近C端,且以HLA-A*02:01、HLA-A*03限制性表位为主;Th表位各表型候选表位肽数目均较多。结论沙眼衣原体MOMP含有潜在的B细胞和T细胞抗原表位,其免疫优势表位主要位于N端42~65 aa、155~185 aa区段和C端342~359 aa区段。Objective To predict and analyze the immunodominant epitopes of major outer membrane protein(MOMP)of Chlamydia trachomatis(Ct).Methods The amino acid sequence of Ct MOMP was retrieved from UniProt protein database,and the B-cell epitopes of MOMP were predicted according to its physical and chemical properties,sec-ondary structure,hydrophilicity parameters,surface accessibility parameters,polarity parameters,antigenicity and flexi-bility parameters.Cytotoxic T lymphocyte epitopes were predicted by SYFPEITHI,IEDB,and Net CTI 1.2 server.Help-er T cell epitopes were predicted by RANKPEP and SYFPEITHI,and the polypeptide fragments with high scores were se-lected as the candidate epitopes.Meanwhile,the three-dimensional structure of MOMP was modeled by Galaxy web.Re⁃sults MOMP was composed of 393 amino acids,mainly random coil andαhelix;it was speculated that B-cell epitopes were located in the N-terminal 42-52aa,161-179aa,259-265aa and 349-358aa segments;its CTL epitopes were mainly concentrated in the proximal C-terminal,and mainly HLA-A*02:01 and HLA-A*03 restricted epitopes.The number of candidate epitope peptides predicted by each phenotype of Th epitope was higher.Conclusion Bioinformatics analysis shows that MOMP contains potential B-cell and T-cell epitopes,and its immunodominant epitopes are mainly located in the N-terminal 42-65aa,155-185aa and C-terminal 342-359aa.

关 键 词：沙眼衣原体 主要外膜蛋白 抗原表位 生物信息学 

分 类 号：R373[医药卫生—病原生物学]