吸食安钠咖对大鼠学习记忆能力的影响及其机制研究  

Effects of Caffeine and Sodium Benzoate on learning and memory ability in rats and its mechanism

作　　者：李建忠[1,2] 郝兴华[3] 吴海平[1,2] 武志兵[1] 张丽芳 Li Jianzhong;Hao Xinghua;Wu Haiping;Wu Zhibing;Zhang Lifang(Department of Human Anatomy,Changzhi Medical College,Changzhi 046000,Shanxi Province,China;Laboratory of Neurodegenerative Diseases,Institute of Basic Medicine,Changzhi Medical College,Changzhi 046000,Shanxi Province,China;Department of Clinical Psychology,Heping Hospital Affiliated to Changzhi Medical College,Changzhi 046000,Shanxi Province,China;Department of Neurology,Changzhi People's Hospital,Changzhi 046099,Shanxi Province,China)

机构地区：[1]长治医学院人体解剖学教研室 [2]长治医学院基础医学研究所神经退行性疾病实验室 [3]长治医学院附属和平医院临床心理门诊,山西长治046000 [4]长治市人民医院神经内科,山西长治046099

出　　处：《中国临床解剖学杂志》2022年第4期432-437,441,共7页Chinese Journal of Clinical Anatomy

基　　金：山西省卫生健康委员会科研项目(2015158;201602027;2020135);山西省高等学校科技创新项目(2019L0676);山西省高等学校创新创业训练项目(2020390)。

摘　　要：目的探讨吸食安钠咖对大鼠学习记忆能力及海马突触可塑性的影响。方法取33只SD大鼠随机分为对照组(C)、安钠咖小剂量组(A-LD)和安钠咖大剂量组(A-HD),每组11只,连续60 d灌胃给药1次/日(C组予生理盐水1 mL,A-LD组予安钠咖溶液60 mg/kg,A-HD组予安钠咖溶液120 mg/kg)。通过Morris水迷宫实验检测大鼠学习记忆能力,免疫组织化学染色和Western blot检测海马突触素(synaptophysin,SYN)和突触后致密蛋白95(postsynaptic density 95,PSD95)表达,Golgi染色检测海马CA1区树突棘密度,透射电镜和体视学方法检测海马CA1区突触数密度(Nv)和面密度(Sv)。结果Morris水迷宫实验显示,与C组比较,A-LD组大鼠逃避潜伏期、目标象限停留时间和穿越平台次数无统计学差异(P>0.05),A-HD组逃避潜伏期明显延长,目标象限停留时间和穿越平台次数明显减少,差异具有统计学意义(P<0.01)。免疫组织化学染色和Western blot结果显示,与C组比较,A-LD组海马SYN和PSD95表达无统计学差异(P>0.05),A-HD组海马SYN和PSD95表达明显减少,差异有统计学意义(P<0.01)。Golgi染色和透射电镜技术结果显示,与C组比较,A-LD组树突棘密度、Nv和Sv无统计学差异(P>0.05),A-HD组树突棘密度、Nv和Sv都明显减少,差异有统计学意义(P<0.01)。结论大剂量吸食安钠咖可降低大鼠海马突触可塑性,导致学习记忆能力受损。Objective To investigate the effects of Caffeine and Sodium Benzoate on learning and memory ability and hippocampal synaptic plasticity in rats.Methods Thirty-three SD rats were randomly divided into a control(C),a low-dose Caffeine and Sodium Benzoate(A-LD)and a high-dose Caffeine and Sodium Benzoate group(A-HD),with 11 rats in each group.They were administered by gavage once a day(C:1 ml of normal saline;A-LD:60 mg/kg of Caffeine and Sodium Benzoate;A-HD:120 mg/kg of Caffeine and Sodium Benzoate)for 60 days.The learning and memory ability was detected by Morris water maze test.The expression of synaptophysin(SYN)and postsynaptic density 95(PSD95)in hippocampus of rats in each group were detected by immunohistochemical staining and Western blot.The dendritic spine density in hippocampal CA1 region was detected by Golgi staining,and the synaptic numerical density(Nv)and surface density(Sv)were detected by transmission electron microscopy and stereological methods.Results Morris water maze test showed that there was no significant difference in escape latency between C group and A-LD group(P>0.05).While the escape latency significantly prolonged and the time spent in target quadrant and number of platform crossings significantly reduced in A-HD group compared with C group(P<0.01).Immunohistochemical staining and Western blot showed that there was no significant difference in the expression of SYN and PSD95 in hippocampus between C group and A-LD group(P>0.05),while the expression of SYN and PSD95 in hippocampus of A-HD group significantly reduced compared with C group(P<0.01).Golgi staining and transmission electron microscopy showed that there was no significant difference in the dendritic spine density,Nv and Sv between C group and A-LD group(P>0.05),while the dendritic spinedensity,Nv and Sv of A-HD group significantly reduced compared with C group(P<0.01).Conclusions The high-dose Caffeine and Sodium Benzoate can reduce the hippocampal synaptic plasticity of rats,resulting in the impairment of learning and mem

关 键 词：安钠咖 学习能力 记忆能力 海马 突触可塑性 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]