精浆SIRT1蛋白表达与患者年龄和精子参数的相关性分析  

Correlation of seminal plasma SIRT1 protein expression level with ages and sperm parameters

作　　者：周芳 顾本宏 韩文均[2] 李柏胜[1] 乐威[3] 拉巴平措 郭建兵 张进[5] 章劲夫[1] ZHOU Fang;GU Benhong;HAN Wenjun;LI Baisheng;LE Wei;Labapingcuo;GUO Jianbing;ZHANG Jin;ZHANG Jinfu(Tongren Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200050,China;Shanghai Seventh People's Hospital,Shanghai 200137,China;Tongji Hospital,Tongji University,Shanghai 200065,China;The People's Hospital of Shigatse City,Shigatse 857000,Tibet,China;Renji Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200032,China)

机构地区：[1]上海交通大学医学院附属同仁医院,上海200050 [2]上海市第七人民医院,上海200137 [3]同济大学附属同济医院,上海200065 [4]日喀则市人民医院,西藏日喀则857000 [5]上海交通大学医学院附属仁济医院,上海200032

出　　处：《检验医学》2022年第6期510-513,共4页Laboratory Medicine

基　　金：上海市自然科学基金项目(19ZR1448700);上海市自然科学基金项目(21ZR1454300)。

摘　　要：目的分析男性精浆中沉默信息调节因子1(SIRT1)蛋白水平与精子参数、年龄的相关性。方法选取上海交通大学医学院附属同仁医院和上海市第七人民医院男科21~40岁男性患者110例,进行精液常规和精浆SIRT1蛋白水平检测。分析年龄、精子参数与精浆SIRT1蛋白水平的相关性。以60例精子参数正常患者为对照组,50例精子参数异常患者为异常组;每组再按照年龄分为21~30岁和31~40岁2个年龄亚组;比较不同精子参数和年龄各组精浆SIRT1蛋白水平。结果精浆SIRT1蛋白水平与年龄、精子浓度、前向运动精子百分率(PR)呈正相关(r值分别为0.28、0.42、0.29,P<0.01)。按年龄比较,所有患者中,异常组精浆SIRT1蛋白水平低于对照组(P<0.01);31~40岁患者中,异常组精浆SIRT1蛋白水平也低于对照组(P<0.05)。不同精子参数患者比较,31~40岁组精浆SIRT1蛋白水平明显高于21~30岁组(P<0.01);对照组中,31~40岁患者精浆SIRT1蛋白表达水平也明显高于21~30岁患者(P<0.01)。结论精浆SIRT1蛋白表达与男性年龄、精子参数明显相关,随精子数量、质量的降低而降低,随年龄的升高而升高,精浆SIRT1蛋白或可作为男性生育潜力的评估指标。Objective To analyze the correlation of seminal plasma silent information regulator 1(SIRT1)protein expression level with sperm parameters and ages.Methods A total of 110 male patients of 21-40-year-old from the andrology departments of Tongren Hospital of Shanghai Jiao Tong University School of Medicine and Shanghai Seventh People's Hospital were enrolled,and their semen analysis and seminal plasma SIRT1 protein expression level were performed and determined.The relation was evaluated among age,sperm parameters and seminal plasma SIRT1 protein expression levels.The control group was composed of 60 patients with normal sperm parameters,and the abnormal group was consisted of 50 patients with abnormal sperm parameters.The research subjects were classified into 21-30-year-old and 31-40-year-old groups.Seminal plasma SIRT1 protein expression levels in these groups were compared.Results The expression level of seminal plasma SIRT1 protein was positively correlated with age,sperm concentration and progressive motility sperm rate(PR)(r values were 0.28,0.42 and 0.29,P<0.01).According to different ages,seminal plasma SIRT1 protein expression level in abnormal group was lower than that in control group(P<0.01).In 31-40-year-old group,seminal plasma SIRT1 protein expression level in abnormal group was lower than that in control group(P<0.05).According to different sperm parameters,seminal plasma SIRT1 protein expression level in 31-40-year-old group was higher than that in 21-30-year-old group(P<0.01),and it of 31-40-year-old patients was also higher than that of 21-30-year-old patients in control group(P<0.01).Conclusions Seminal plasma SIRT1 protein expression is related to ages and sperm parameters,decreasing with sperm quantity and quality and increasing with age,which could be utilized to assess male reproductive potential.

关 键 词：沉默信息调节因子1 精子参数 年龄 

分 类 号：R446.1[医药卫生—诊断学]