阴地翠雀花的化学成分研究  

Study on the chemical constituents of Delphinium umbrosum Hand.-Mazz.

作　　者：郭秋菊 杨传轮 陈琳[1] 黄帅[1] 郭春生 张纯姑 GUO Qiu-ju;YANG Chuan-lun;CHEN Lin;HUANG Shuai;GUO Chun-sheng;ZHANG Chun-gu(School of Life Science and Engineering,Southwest Jiaotong University;School of Physic Science and Technology,Southwest Jiaotong University,Chengdu 610031,China;Chambroad Chemical Industry Research Institute Co.,Ltd.,Binzhou 256500,China)

机构地区：[1]西南交通大学生命科学与工程学院 [2]西南交通大学物理科学与技术学院,成都610031 [3]黄河三角洲京博化工研究院有限公司,滨州256500

出　　处：《天然产物研究与开发》2022年第7期1148-1155,共8页Natural Product Research and Development

基　　金：国家自然科学基金(21807089);四川省应用基础项目(2021YJ0222);教育部产学合作协同育人项目(202102414025,202102414026)。

摘　　要：为研究阴地翠雀花(Delphinium umbrosum Hand.-Mazz.)的化学成分,采用硅胶柱色谱从其95%乙醇提取物中分离得到14个化合物。通过HR-ESI-MS、1D和2D NMR等波谱技术鉴定了它们的结构,包括13个牛扁碱型C19-二萜生物碱:牛扁碱(1)、氨茴酰牛扁碱(2)、majusine A(3)、14-deacetylajadine(4)、ajacine(5)、14-deacetylnudicauline(6)、甲基牛扁碱(7)、德尔色明A和B(8)、delavaine A free acid和delavaine B free acid(9)、德拉瓦印A和B(10)、拉翠碱A和B(11)、umbrosumine C(12)、umbrosumines A和B(13),以及1个异喹啉类生物碱:S-glaucine(14)。所有化合物均为首次从该植物中分离得到。测试了化合物(3~8、10~13)对脂多糖诱导小鼠RAW 264.7巨噬细胞产生NO的抑制作用,以及化合物1~14对小鼠乳腺癌4T1细胞的抗肿瘤作用,结果显示所有测试化合物均无明显抗炎及抗肿瘤活性。To study the chemical constituents of Delphinium umbrosum Hand.-Mazz.,fourteen compounds were isolated from the ethanol extract of D.umbrosum by silica gel column chromatography.Their structures were identified by means of spectroscopic methods such as HR-ESI-MS,1D and 2D NMR,including thirteen lycoctonine C19-type diterpenoid alkaloids:lycoctonine(1),anthranoyllycoctonine(2),majusine A(3),14-deacetylajadine(4),ajacine(5),14-deacetylnudicauline(6),methyllycaconitine(7),delsemines A and B(8),delavaine A free acid and delavaine B free acid(9),delavaines A and B(10),gyalanines A and B(11),umbrosumine C(12),umbrosumines A and B(13),and one isoquinoline alkaloid:S-glaucine(14).All compounds were isolated from this plant for the first time.The inhibitory effects of compounds(3-8,10-13)on lipopolysaccharide-induced NO production in mouse RAW 264.7 macrophages,and the anti-tumor effects of compounds 1-14 on mouse breast cancer 4T1 cells were tested,and the results showed that all the tested compounds had no obvious anti-inflammatory and anti-tumor activities.

关 键 词：阴地翠雀花 二萜生物碱 抗炎 抗肿瘤 

分 类 号：R284.1[医药卫生—中药学]