溴隐亭对斑马鱼胚胎早期发育的影响  

Effects of bromocriptine on early development of zebrafish embryos

作　　者：林飞阳 吴成坤 陈兵 龙霄翱 刘思微 黄俊章 黄煌 文涛 余庆旺 刘豪然 廖壮槟 LIN Fei-Yang;WU Cheng-Kun;CHEN Bing;LONG Xiao-Ao;LIU Shi-Wei;HUANG Jun-Zhang;HUANG Huang;WEN Tao;YU Qing-Wang;LIU Hao-Ran;LIAO Zhuang-Bin(Department of Neurosurgery,Affiliated Hospital of Guangdong Medical University,Zhanjiang,Guangdong 524001,China)

机构地区：[1]广东医科大学附属医院神经外科,广东湛江524001

出　　处：《国际神经病学神经外科学杂志》2022年第3期8-13,共6页Journal of International Neurology and Neurosurgery

基　　金：2021年度广东医科大学青年培育基金项目(GDMUQ2021027)。

摘　　要：目的探讨溴隐亭对斑马鱼胚胎生存率、孵化时间和形态发育异常的影响。方法取斑马鱼胚胎随机分成溴隐亭溶液组(10μmol/L组、20μmol/L组、50μmol/L组、100μmol/L组),control组(空白对照组)和0.5%二甲基亚砜(DMSO)对照组,比较各组胚胎生存率、孵化时间、幼鱼鱼鳔充气、幼鱼畸形及发育异常情况。结果48受精后发育小时数(HPF)时,溴隐亭溶液组胚胎孵化率低于2个对照组(P<0.05);120 HPF时,20μmol/L组、50μmol/L组、100μmol/L组斑马鱼幼鱼鱼鳔充气率、水肿率分别低于、高于其余组(P<0.05),但各组畸形率比较,差异无统计学意义(P>0.05);144 HPF时,50μmol/L组、100μmol/L组较其余4组斑马鱼幼鱼生存率降低(P<0.05)。结论高浓度溴隐亭可引起斑马鱼胚胎孵化延迟、斑马鱼幼鱼生存率降低、水肿及鱼鳔充气障碍,但对斑马鱼胚胎发育畸形则无影响。Objective To explore the effects of bromocriptine on the survival rate,hatching time,and morphological development of zebrafish embryos.Methods Zebrafish embryos were randomly divided into bromocriptine solution groups(10-μmol/L,20-μmol/L,50-μmol/L,and 100-μmol/L groups),blank control group,and 0.5%dimethyl sulfoxide control group.These groups were compared with regard to the survival rate,hatching time,swim bladder inflation,and deformities and anomalies.Results At 48 hours post fertilization(HPF),the bromocriptine groups showed significantly lower hatching rates than the two control groups(all P<0.05).At 120 HPF,the 20-μmol/L,50-μmol/L,and 100-μmol/L bromocriptine groups showed significantly lower swim bladder inflation rates and significantly higher edema rates than the other groups(all P<0.05),but without significant differences in the rate of deformities between groups(all P>0.05).At 144 HPF,the survival rate of zebrafish larvae was significantly lower in the 50-μmol/L and 100-μmol/L bromocriptine groups than in the other four groups(all P<0.05).Conclusion High concentrations of bromocriptine can delay embryos’hatching,reduce larvae’s survival rate,and cause edema and impaired swim bladder inflation in zebrafish,but have no association with the developmental deformities of zebrafish embryos.

关 键 词：垂体催乳素腺瘤 溴隐亭 多巴胺受体激动剂 斑马鱼 胚胎发育 致畸作用 

分 类 号：R971[医药卫生—药品]