参芍口服液对急性心肌梗死大鼠的心肌保护作用分析  

Effect of Shenshao Oral Liquid on Myocardial Protection of Rats with Acute Myocardial Infarction

作　　者：刘帆 邸亚丽[1] 马威[1] 李霞[1] 杨立明[1] 亢小丽[1] 郭月 纪征[1] LIU Fan;DI Ya-li;MA Wei;LI Xia;YANG Li-ming;KANG Xiao-li;GUO Yue;JI Zheng(The First Department of Cardiology,Tangshan Workers'Hospital,Tangshan,Hebei 063000,China)

机构地区：[1]唐山市工人医院心内一科,河北唐山063000

出　　处：《解放军医药杂志》2022年第6期1-6,共6页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：河北省中医药管理局科学研究计划项目(2020429)。

摘　　要：目的探究参芍口服液对急性心肌梗死(AMI)大鼠的心肌保护作用及机制。方法将60只SD大鼠随机分为假手术组、模型组及低、中、高剂量(3.2、6.4、12.8 ml/d)参芍口服液组和阳性对照组[贝那普利10 mg/(kg·d)],除假手术组外,其余大鼠均手术结扎冠状动脉制备AMI模型,假手术组仅开胸未结扎,药物干预4周后,行超声心动图评估各组大鼠左心室功能和结构,HE染色观察各组大鼠心肌组织病理学变化,比较各组大鼠血清肌酸激酶(CK)、心肌型肌酸激酶同工酶(CK-MB)、脑钠肽(BNP)水平,ELISA检测外周血白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平,行免疫组织化学染色和Western blot实验检测各组大鼠心肌组织Wnt10b、β-catenin蛋白表达。结果与模型组比较,中、高剂量参芍口服液组和阳性对照组大鼠心肌组织病理损伤有不同程度改善,左心室射血分数、左心室短轴缩短率升高,左心室舒张末期内径、左心室收缩末期内径、左心室舒张末期容积和左心室收缩末期容积及血清CK、CK-MB、BNP、IL-1β、IL-6、TNF-α水平均降低,心肌组织Wnt10b、β-catenin蛋白表达显著降低(P<0.05)。结论参芍口服液可能通过调控Wnt/β-catenin信号通路,改善AMI大鼠心室重构和心功能,减轻炎症反应,促进心肌组织修复与再生,对心肌发挥保护作用。Objective To explore the myocardial protective effect and mechanism of Shenshao Oral Liquid on rats with acute myocardial infarction(AMI).Methods A total of 60 SD rats were randomly divided into sham operation group,model group,low,medium and high-dose(3.2,6.4,12.8 ml/d)Shenshao Oral Liquid groups and positive control group[Benazepril 10 mg/(kg·d)].Except for sham operation group,coronary arteries of rats in the other groups were ligated to prepare AMI models.In sham operation group,thoracotomy was performed without ligation.After drug intervention for 4 weeks,the left ventricular function and structure were evaluated by performing echocardiography,and histopathological changes of myocardial tissues were observed by HE staining,and levels of serum creatine kinase(CK),myocardial creatine kinase isoenzyme(CK-MB)and brain natriuretic peptide(BNP)were compared in each group.Levels of interleukin-1β(IL-1β),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in peripheral blood were detected by enzyme linked immunosorbent assay(ELISA),and immunohistochemical staining and Western blot were used to detect Wnt10b andβ-catenin protein expressions.Results Compared with those in model group,pathological injuries of myocardial tissues were significantly improved in varying degrees;left ventricular ejection fraction and left ventricular shortening fraction were significantly increased,while levels of left ventricular end diastolic dimension,left ventricular end systolic dimension,left ventricular end diastolic volume,left ventricular end systolic volume and serum levels of CK,CK-MB,BNP and IL-1β,IL-6 and TNF-αwere significantly decreased,and the expressions of Wnt10b andβ-Catenin protein were significantly decreased in medium and high-dose Shenshao Oral Liquid groups and positive control group(P<0.05).Conclusion Shenshao Oral Liquid may improve ventricular remodeling and cardiac function in AMI rats,reduce inflammatory reaction,promote repair and regeneration of myocardial tissues by regulating Wnt/β-catenin signali

关 键 词：心肌梗死 参芍口服液 心肌损伤 心功能 WNT/Β-CATENIN信号通路 大鼠 Sprague-Dawley 

分 类 号：R542.22[医药卫生—心血管疾病] R-332[医药卫生—内科学]