嗅闻佛手柑精油对阿尔兹海默症小鼠的神经行为的影响  

Effects of sniffing bergamot essential oil on neurobehavior in mice with Alzheimer’s disease

作　　者：胡慧艳 文志强 任婧婻 李晓 范刚 潘思轶 张璐璐 HU Huiyan;WEN Zhiqiang;REN Jingnan;LI Xiao;FAN Gang;PAN Siyi;ZHANG Lulu(College of Food Science and Technology,Huazhong Agricultural University/Key Laboratory of Environment Correlative Dietology,Ministry of Education/Hubei Key Laboratory of Fruit&Vegetable Processing&Quality Control,Wuhan 430070,China;Hubei Province Rearing Fine Breed Livestock Farm,J ingzhou 434010,China;College of Food Science and Technology,Henan University of Technology,Zhengzhou 450001,China)

机构地区：[1]华中农业大学食品科学技术学院/环境食品学教育部重点实验室/果蔬加工与品质调控湖北省重点实验室,武汉430070 [2]湖北省畜牧良种场,荆州434010 [3]河南工业大学粮油食品学院,郑州450001

出　　处：《华中农业大学学报》2022年第4期151-157,共7页Journal of Huazhong Agricultural University

基　　金：中央高校基本科研业务费专项(2662020SPPY002);湖北省重点研发计划项目(2020BBA049)。

摘　　要：为研究嗅闻不同浓度佛手柑精油对D-gal和AlCl3联合诱导的阿尔兹海默症模型小鼠的神经保护作用,选用健康的雄性昆明小鼠,通过腹腔注射D-gal(120 mg/kg)和AlCl3(10 mg/kg)持续49 d以建立阿尔兹海默症实验模型,第29天起,嗅闻试验组小鼠嗅闻25%或75%佛手柑精油,每天1 h,持续21 d,嗅闻试验结束后评估小鼠行为和大脑生物化学的变化。行为学实验结果显示,嗅闻佛手柑精油显著改善了阿尔兹海默症模型小鼠的认知障碍和探索性下降等症状。生化实验结果显示,嗅闻75%佛手柑精油明显抑制了氧化应激的程度和乙酰胆碱酶活性的增加,促进了小鼠认知能的提高。结果表明,嗅闻75%佛手柑精油可以减轻D-gal和AlCl3引发的小鼠认知行为障碍和生化损伤。Bergamot essential oil(BEO)is known for having many health benefits.Healthy male kunming mice were selected to establish the model mice with Alzheimer’s disease by intraperitoneal injection of D-gal(120 mg/kg)and AlCl_(3)(10 mg/kg)for 49 days to study the neuroprotective effect of sniffing different concentrations of bergamot essential oil on the model mice with Alzheimer’s disease induced by the combination of D-gal and AlCl_(3).From the 29th day,the mice in the sniffing group sniffed 25%or 75%bergamot essential oil for 21 days,1 h everyday.After the sniffing experiment,the changes in the behavior and brain biochemistry of the mice were evaluated.The results of behavioral studies showed that sniffing bergamot essential oil significantly improved symptoms including cognitive impairment and exploratory decline in the model mice with Alzheimer’s disease.The results of biochemical studies showed that sniffing 75%bergamot essential oil significantly inhibited the degree of oxidative stress and the increase of acetylcholinesterase(AchE)activity,and promoted the improvement of cognitive function in mice.It is indicated that sniffing 75%bergamot essential oil attenuates D-gal and AlCl_(3)-induced cognitive-behavioral impairment and biochemical damage.

关 键 词：佛手柑精油 阿尔兹海默症 氧化应激 乙酰胆碱酶 芳香疗法 神经保护 

分 类 号：R285[医药卫生—中药学] R277.7[医药卫生—中医学]