转运蛋白MoMfs2对稻瘟病菌细胞壁完整性和杀菌剂外排的调控  

Regulation of MFS transporter MoMfs2 on cell wall integrity and discharge of fungicides of Magnaporthe oryzae

作　　者：齐中强[1] 杜艳[1] 潘夏艳 文友义 于俊杰[1] 张荣胜[1] 俞咪娜[1] 曹慧娟 宋天巧[1] 李连伟 刘永锋[1] QI Zhong-qiang;DU Yan;PAN Xia-yan;WEN You-yi;YU Jun-jie;ZHANG Rong-sheng;YU Mi-na;CAO Hui-juan;SONG Tian-qiao;LI Lian-wei;LIU Yong-feng(Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;School of Life Sciences Jiangsu Normal University,Xuzhou,Jiangsu 221116,China)

机构地区：[1]江苏省农业科学院植物保护研究所,南京210014 [2]江苏师范大学生命科学学院,江苏徐州221116

出　　处：《西南农业学报》2022年第6期1325-1332,共8页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金面上项目(31871921);江苏省农业自主创新资金(CX19[1008]);江苏现代农业稻麦产业技术体系(JAST[2021]271);江苏省自然科学基金青年基金项目(BK20181005)。

摘　　要：【目的】稻瘟病菌(Magnaporthe oryzae)引起的稻瘟病是水稻生产上一种最具破坏性的真菌病害,严重威胁我国的水稻生产安全。研究转运蛋白MoMfs2编码基因MoMFS2(MGG_01301)在稻瘟病菌生长发育及致病过程中的功能,为开发新型杀菌剂提供参考。【方法】利用基因敲除策略将其进行缺失突变并对突变体进行了一系列的生物学表型分析。【结果】实验显示该突变体无论在自然培养基还是在合成培养基上,生长速率较野生型均没有变化,表明MoMFS2基因不参与稻瘟病菌对营养的吸收利用;另外,该突变体原生质体释放速度变慢,对细胞壁胁迫因子刚果红和SDS耐受性显著增强,表明该基因参与了对细胞壁胁迫的应答,进一步推测其在稻瘟病菌细胞壁完整性发挥重要作用。此外,该突变体不能在含有刚果红和ABTS平板上产生晕圈,同时其对胞外漆酶和过氧化物酶的分泌显著降低,表明MoMfs2参与了对胞外漆酶和过氧化物酶的分泌;另外该突变体对不同类型的杀菌剂(咪鲜胺、多菌灵、和嘧菌酯)敏感性显著增强,表明该突变体对杀菌剂的耐受性降低,推测MoMFS2基因参与了稻瘟病菌对杀菌剂的外排。通过水稻喷雾和大麦离体致病性测定发现该突变体对水稻和大麦的致病性较野生型没有变化,表明MoMFS2基因不参与稻瘟病菌的致病性。【结论】转运蛋白MoMfs2参与调控稻瘟病菌细胞壁完整性和对胞外漆酶和过氧化物酶的分泌及对杀菌剂的外排,不参与稻瘟病菌的致病性。【Objective】Rice blast caused by Magnaporthe oryzae was one of the most destructive fungal diseases in rice production,which seriously threatens the safety of rice production in China.The study on the function of the transporter MoMfs2 encoding gene MoMFS2(MGG_01301)in the growth,development and pathogenicity of M.oryzae provided a reference for the development of new fungicides.【Method】The deletion mutation of MoMFS2 was carried out using a knockout strategy and a series of biological phenotypes of the mutant were analyzed.【Result】The growth rate of the mutant was not different from that of the wild type on natural medium or synthetic medium,which indicated that MoMFS2 was not involved in the absorption and utilization of nutrition of M.oryzae.Moreover,the release rate of protoplast of the mutant was slow,the tolerance to cell wall stress congo red and SDS was significantly enhanced,which indicated that was involved in the response to cell wall stress and further speculated that it played an important role in cell wall integrity of M.oryzae.In addition,the mutant could not produce halo on CM medium containing Congo red and ABTS,and the secretion of extracellular laccase and peroxidase of the mutant was significantly decreased,indicating that MoMfs2 was involved in the secretion of extracellular laccase and peroxidase.On the other hand,the mutant was more sensitive to different types of fungicides(imidamide,carbendazim and pyrimethanil),which suggested that the mutant had reduced tolerance to fungicides,and speculated that MoMFS2 was involved in fungicidal efflux of M.oryzae.The pathogenicity of the mutant to rice and barley was no different from that of the wild type,indicating that MoMFS2 was not involved in pathogenicity of M.oryzae.【Conclusion】The transporter MoMfs2 was involved in the regulation of cell wall integrity,secretion of extracellular laccase and peroxidase and discharge of fungicides,but not in pathogenicity of M.oryzae.

关 键 词：稻瘟病菌 MoMfs2 细胞壁完整性 杀菌剂 外排 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]