1株鸽毛滴虫的分离鉴定、体外培养和感染动物模型的建立  

Isolation,identification,culture in vitro and establishment of infection animal model of Trichomonas gallinae

作　　者：丁聪 翟涛 李玲 王晓岑 宫鹏涛 张西臣 李建华 李新 DING Cong;ZHAI Tao;LI Ling;WANG Xiaocen;GONG Pengtao;ZHANG Xicheng;LI Jianhua;LI Xin(College of Veterinary Medicine,Jilin University,Changchun 130062,China)

机构地区：[1]吉林大学动物医学学院,吉林长春130062

出　　处：《中国兽医学报》2022年第5期926-932,共7页Chinese Journal of Veterinary Science

基　　金：中国博士后科学基金资助项目(2019M651213)。

摘　　要：分离和培养鸽毛滴虫,并建立鸽毛滴虫感染动物模型。采集并研磨患毛滴虫病的落地王鸽脑组织,用RPMI1640和牛肝浸汤培养基进行体外培养;光学及电子显微镜下观察虫体形态和超微结构;对ITS1/5.8S/ITS2基因序列进行系统发育分析及限制性内切酶片段长度多态性分析确定其基因型;以不同剂量虫体经口感染鸽,连续5 d,通过口咽拭子镜检感染率和口咽部剖检病变观察等建立鸽毛滴虫感染动物模型。结果显示,从病鸽脑组织中分离出一株鸽毛滴虫,鉴定为基因A型。攻虫量1×10^(6)个虫体后,鸽口咽部剖检可见黄色黏液或黄白色干酪样物质,口咽拭子镜检感染率为80%。本研究为鸽毛滴虫致病机制、药物筛选等研究奠定了良好基础。A strain of Trichomonas gallinae was isolated and cultured,and the animal model of this Trichomonas gallinae infection was established.The brain tissues of king pigeons with trichomoniasis were collected and ground,then cultured in vitro with RPMI 1640 and bovine liver soaking soup medium.The isolated parasite was observed under light and electron microscope for its morphology and ultrastructure.To determine the genotype,this parasite was identified by phylogenetic analysis using the ITS1/5.8 S/ITS2 gene sequence,and restriction fragment length polymorphism analysis.In order to establish the animal model of the Trichomonas gallinae infection in pigeons,king pigeons were infected orally with different doses of this parasite for 5 d,the infection rates were detected by the oral and pharyngeal swabs with microscopic examination,and pathological changes of oropharynx were observed.The results showed that a strain of Trichomonas gallinae was cultured in vitro and isolated,and identified as gene type A.After infected with 1×10^(6)Trichomonas gallinae,pigeons were found with yellow mucus or yellow-white caseous substances in the oropharynx,and the infection rate of oral and pharyngeal swabs by microscopy was 80%.These data can lay a good foundation for the study of the pathogenic mechanism and drug screening of Trichomonas gallinae.

关 键 词：鸽毛滴虫 体外培养 形态观察 基因型 感染模型 

分 类 号：S855.9[农业科学—临床兽医学]