β-羟基丁酸增强奶牛中性粒细胞的黏附  

β-hydroxybutyric acid enhances adhesion of bovine neutrophils

作　　者：何纪元 李心慰 王哲 刘国文 宋玉祥 HE Jiyuan;LI Xinwei;WANG Zhe;LIU Guowen;SONG Yuxiang(College of Veterinary Medicine,Jilin University,Changchun 130062,China)

机构地区：[1]吉林大学动物医学学院,吉林长春130062

出　　处：《中国兽医学报》2022年第5期993-998,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金青年科学基金资助项目(JJKH20211135KJ)。

摘　　要：奶牛亚临床酮病(subclinical ketosis,SCK)是一种围产期高产奶牛高发的代谢紊乱性疾病,伴有高酮血症和系统性炎性反应。中性粒细胞(polymorphonuclear granulocyte,PMN)在调控系统性炎症反应中起重要作用。β-羟基丁酸(β-hydroxybutyric acid,BHBA)可能通过增强PMN黏附提高其促炎能力,进而导致系统性炎性反应的发生,然而其调控机制尚不清楚。本研究根据血清BHBA水平筛选出对照组(CON,n=15,BHBA<0.6 mmol/L,Glu>3.0 mmol/L)和SCK组(n=15,1.2 mmol/L<BHB<3.0 mmol/L,Glu<2.8 mmol/L)。奶牛血液免疫生化指标结果显示:SCK奶牛血清白介素1β(interleukin-1,IL-1β)、白介素6(interleukin-6,IL-6)、肿瘤坏死因子(tumor necrosis factor,TNF-α)、触珠蛋白(haptoglobin,HP)和血清淀粉样蛋白(serum amyloid A,SAA)的浓度均显著增加。体外使用BHBA刺激健康奶牛分离的PMN显著增强了其黏附能力,显著增加了黏附相关分子淋巴细胞功能相关抗原-1(lymphocyte function association antigen-1,CD11a)和巨噬细胞功能相关抗原-1(macrophage-1,CD11b)的蛋白丰度;显著增加了炎性因子TNF-α、IL-1β和IL-6的mRNA丰度。以上数据表明,BHBA能够增加奶牛PMN黏附相关分子的表达,进而增强其黏附能力和促炎功能,为SCK奶牛的系统性炎性反应的发生和发展机制提供了新的理论解释。Subclinical ketosis(SCK)is a metabolic disorder with a high incidence in perinatal high-yielding dairy cows,which is accompanied by hyperkalemia and systemic inflammatory reaction.Polymorphonuclear granulocyte(PMN)plays an important role in regulating the systemic inflammatory response.β-Hydroxybutyric acid(BHBA)may enhance PMN adhesion,improve its ability to produce pro-inflammatory cytokines,leading to the systemic inflammatory response.However,the underlying regulatory mechanism is still unclear.Here,cows were divided as control group(n=15,BHBA<0.6 mmol/L)or SCK(n=15,1.2 mmol/LL<BHBA<3.0mmol/L)group based on serum BHBA concentration.The results showed that the serum concentration of interleukin 1B(IL-1B),interleukin 6(IL-6),tumor necrosis factor(TNF-a),haptoglobin(HP),and serum amyloid(SAA)were greater in SCK cows.In uitro,BHBA enhanced the adhesion of PMN isolated from healthy cows.The protein abundance of lymphocyte function association antigen-1(CD11a)and macrophage-1(CD11b)were greater after BHBA treatment.The mRNA abundance of inflammatory factors including IL-1B,IL-6 and TNF-a were greater after BHBA treatment.The above datashowed that BHBA can increase the expression of adhesion molecules in bovine PMN,thus enhancing their adhesion and pro-inflammatory functions Our data provide anew explanation for the development of systemic inflammatory response in SCK.

关 键 词：奶牛 亚临床酮病 β-羟基丁酸 中性粒细胞 系统性炎性反应 

分 类 号：S852.3[农业科学—基础兽医学] S823.91[农业科学—兽医学]