云南省异烟肼耐药结核分枝杆菌katG和inhA基因突变特征  

Characteristics of katG and inhA Gene Mutations in INHResistant Mycobacterium Tuberculosis in Yunnan Province

作　　者：陈连勇[1] 茹浩浩 杨星[1] 闫双群[1] 陈涛 倪沁璇 许琳[1] CHEN Lianyong;RU Haohao;YANG Xing;YAN Shuangqun;CHEN Tao;NI Qinxuan;XU Lin(Dispensary for Tuberculosis Control and Prevention,Yunnan Center for Disease Control and Prevention,Kunming Yunnan 650022,China)

机构地区：[1]云南省疾病预防控制中心结核病防治所,云南昆明650022

出　　处：《昆明医科大学学报》2022年第8期28-33,共6页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(81560544)。

摘　　要：目的分析云南省异烟肼耐药结核分枝杆菌katG和inhA基因突变特征。方法采用PCR方法对云南省异烟肼耐药结核分枝杆菌进行katG和inhA基因扩增,并将扩增产物进行基因测序后比对分析。结果88株异烟肼耐药菌株中,耐药基因与表型药敏结果的符合率为82.95%(73/88),katG和inhA基因突变率分别为75.00%(66/88)和9.09%(8/88)。最为常见的基因突变位点为katG315(67.05%)和inhA-15(7.95%),katG315位点基因突变主要表现为Ser315Thr(59.09%,52/88)。耐多药与单耐异烟肼菌株中katG基因突变比例,差异有统计学意义(χ^(2)=4.190,P=0.041),而inhA基因及katG315、inhA-15位点基因突变比例,差异无统计学意义(P>0.05)。结论云南省异烟肼耐药以katG315和inhA-15基因突变为主,MDR菌株中katG基因突变率高于异烟肼单耐,目前基于基因突变的分子检测技术是检测异烟肼耐药的有效手段。Objective To analyze the characteristics of katG and inhA genetic mutations associated with INH-resistant Mycobacterium tuberculosis in Yunnan Province.Methods The INH-resistant Mycobacterium tuberculosis isolates were collected from drug-resistant monitoring sites in Yunnan Province,and the katG and inhA genes were amplified by PCR and then sequenced.Results Among the 88 MTB strains,the coincidence rate of INH-resistant genotype and phenotype was 82.95%(73/88).75.00%(66/88)had katG mutations and 9.09%(8/88)had inhA mutations.katG315(67.05%)and inhA-15(7.95%)were the top two mutation sites,and a high prevalence of katG Ser315Thr mutations(59.09%,52/88)was observed.The frequency of the katG mutation was significantly higher in MDR-TB compared to INH mono-resistant isolates(χ^(2)=4.190,P=0.041),while katG315,inhA and inhA-15T mutations did not differ significantly between these two groups(P>0.05).Conclusion The most common mutation of INH-resistant Mycobacterium tuberculosis in Yunnan Province were katG315 and inhA-15,and katG mutation was higher among MDR isolates compared with INH mono-resistant strains.Molecular diagnostics based on gene mutations was a useful method for rapid detection of INH-resistant Mycobacterium tuberculosis in Yunnan Province.

关 键 词：结核分枝杆菌 异烟肼 耐药 KATG INHA 

分 类 号：R521[医药卫生—内科学] R378.911[医药卫生—临床医学]