Anthrahydroquinone-2,6-disulfonate alleviates paraquat-induced kidney injury via the apelin-APJ pathway in rats  

作　　者：Qi Li Bo Wang Kai-Wen Lin Tang Deng Qi-Feng Huang Shuang-Qin Xu Hang-Fei Wang Xin-Xin Wu Nan Li Yang Yi Ji-Chao Peng Yue Huang Jin Qian Xiao-Ran Liu 

机构地区：[1]College of Emergency and Trauma,Hainan Medical University,Key Laboratory of Emergency and Trauma of Ministry of Education,The First Affiliated Hospital of Hainan Medical University,Haikou 571199,China [2]Jiangdong Health Center,Meilan District,Haikou 571126,China [3]Hainan Women and Children's Medical Center,Haikou 570312,China

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2022年第8期333-342,共10页亚太热带生物医学杂志（英文版）

基　　金：National Natural Science Foundation of China(No.81960351);Social Development Key Project of Hainan Province(No.ZDYF2019125);Hainan Provincial Natural Science Foundation of China(820QN398)Hainan Province Clinical Medical Center.

摘　　要：Objective:To explore the protective effects of anthrahydroquinone-2,6-disulfonate(AH_(2)QDS)on the kidneys of paraquat(PQ)poisoned rats via the apelin-APJ pathway.Methods:Male Sprague Dawley rats were divided into four experimental groups:control,PQ,PQ+sivelestat,and PQ+AH_(2)QDS.The PQ+sivelestat group served as the positive control group.The model of poisoning was established via intragastric treatment with a 20%PQ pesticide solution at 200 mg/kg.Two hours after poisoning,the PQ+sivelestat group was treated with sivelestat,while the PQ+AH_(2)QDS group was given AH_(2)QDS.Six rats were selected from each group on the first,third,and seventh days after poisoning and dissected after anesthesia.The PQ content of the kidneys was measured using the sodium disulfite method.Hematoxylin-eosin staining of renal tissues was performed to detect pathological changes.Apelin expression in the renal tissues was detected using immunofluorescence.Western blotting was used to detect the expression levels of the following proteins in the kidney tissues:IL-6,TNF-α,apelin-APJ(the apelin-angiotensin receptor),NF-κB p65,caspase-1,caspase-8,glucose-regulated protein 78(GRP78),and the C/EBP homologous protein(CHOP).In in vitro study,a PQ toxicity model was established using human tubular epithelial cells treated with standard PQ.Twenty-four hours after poisoning,sivelestat and AH_(2)QDS were administered.The levels of oxidative stress in human renal tubular epithelial cells were assessed using a reactive oxygen species fluorescence probe.Results:The PQ content in the kidney tissues of the PQ group was higher than that of the PQ+AH_(2)QDS group.Hematoxylin-eosin staining showed extensive hemorrhage and congestion in the renal parenchyma of the PQ group.Vacuolar degeneration of the renal tubule epithelial cells,deposition of crescent-like red staining material in renal follicles,infiltration by a few inflammatory cells,and a small number of cast formation were also observed.However,these pathological changes were less severe in the PQ+s

关 键 词：Paraquat poisoning AH2QDS APELIN/APJ Acute kidney injury Oxidative stress Rat Human tubular epithelial cell 

分 类 号：R692[医药卫生—泌尿科学]