大黄素对早期糖尿病肾病大鼠足细胞上皮间质转化的影响  

Effect of emodin on the epithelial-mesenchymal transition of podocytes in early diabetic nephropathy rats

作　　者：李杭霖 杨丽娟[2] 刘磊[1] Li Hanglin;Yang Lijuan;Liu Lei(Department of Nephrology,the First Affiliated Hospital of Bengbu Medical College,Bengbu 233004,China;Department of Physiology,Bengbu Medical College,Bengbu 233030,China)

机构地区：[1]蚌埠医学院第一附属医院肾内科,蚌埠233004 [2]蚌埠医学院生理学教研室,蚌埠233030

出　　处：《中华解剖与临床杂志》2022年第6期418-425,共8页Chinese Journal of Anatomy and Clinics

基　　金：安徽省高校自然科学研究重点项目(KJ2019A0321);蚌埠医学院自然科学重点项目(BYKY2019008ZD)。

摘　　要：目的探讨大黄素对早期糖尿病肾病(DN)大鼠足细胞上皮间质转化(EMT)的影响。方法选取健康雄性SD大鼠40只,按数字表法随机分为对照组10只和实验组30只。对照组大鼠实验过程中采用普通饲料喂养。实验组30只大鼠采用高糖、高脂饮食喂养4周后,一次性腹腔注射链脲佐菌素(40 mg/kg)的方法,制备DN模型,取造模成功的大鼠随机分为DN组和大黄素治疗组(DN+大黄素组)。造模成功后,DN组继续高糖、高脂饮食;DN+大黄素组给予高糖、高脂饮食,联合大黄素(20 mg/kg)灌胃,每天1次,连续8周。各组大鼠在实验14周末观测以下指标:(1)收集24 h尿液,测定24 h尿蛋白量;(2)完成尿液收集后称体质量并记录;(3)处死后取腹主动脉血检测肾肌酐、尿素氮、血糖;(4)取左侧肾脏,苏木精-伊红(HE)染色、过碘酸希夫染色(PAS)观察肾脏病理改变,免疫组织化学染色观察Nephrin、Desmin蛋白的表达情况;(5)取右侧肾脏肾皮质部位组织,电镜下观察足细胞及基底膜变化情况。结果实验组大鼠30只,DN大鼠模型造模成功24只,实验过程中DN组及DN+大黄素组大鼠各死亡2只。(1)14周末对照组、DN组、DN+大黄素组大鼠体质量分别为(350.1±14.16)、(265.2±7.62)、(312.1±11.77)g,差异有统计学意义(F=136.50,P<0.001)。(2)对照组肾肌酐、尿素氮、血糖、24 h尿蛋白分别为(28.88±4.36)μmol/L、(8.76±0.50)mmol/L、(9.81±0.86)mmol/L、(3.60±0.79)mg;DN组分别为(92.30±7.30)μmol/L、(29.41±6.67)mmol/L、(25.10±4.10)mmol/L、(35.23±4.07)mg;DN+大黄素组分别为(52.10±5.80)μmol/L、(14.93±1.03)mmol/L、(16.51±1.14)mmol/L、(13.35±1.89)mg。与对照组比较,DN组、DN+大黄素组肾肌酐、尿素氮、血糖、24 h尿蛋白量均升高;与DN组比较,DN+大黄素组各项指标均降低:差异均有统计学意义(P值均<0.01)。(3)HE染色观察肾组织形态学:对照组肾小球及肾小管结构未见异常;DN组肾小球硬化,肾小囊狭窄,部分肾小�Objective To explore the effect of emodin on podocyte epithelial-mesenchymal transition(EMT)in early diabetic nephropathy(DN)rats.Methods Forty adult healthy male Sprague-Dawley(SD)rats were randomly divided into control(n=10)and experimental(n=30)groups.Rats in the control group were fed with an ordinary diet during the experiment whereas those in the experimental group were fed with a high-glucose and high-fat diet combined with one-time intraperitoneal injection of streptozotocin(40 mg/kg)for 4 weeks to prepare the DN model.Mold success rats were randomly divided into DN and emodin treatment(DN+emodin)groups.The DN group continued to receive the high-fat and high-sugar diet,while the DN+emodin group was given a high-fat and high-sugar diet treated with emodin(20 mg/kg)once a day for 8 weeks.Rats in each group observed the following indexes at the end of the experimental 14 weeks:(1)Collect 24-hour urine volume of rats.Urine protein concentration of rats in 24 hours was calculated using urine protein quantitative test kit.(2)Weigh and record body weight after urine collection.(3)Extract blood of abdominal aorta to detect renal serum creatinine(SCr),serum urea nitrogen(BUN),and blood glucose(BG).(4)Select the left kidney for hematoxylin and eosin(HE)and periodic acid-Schiff(PAS)staining.Immunohistochemistry was applied to observe the expression of Nephrin and Desmin proteins.(5)Collect the renal cortex of the right kidney and observe changes of podocytes and glomerular basement membrane(GBM)under an electron microscope.Results The experimental group was composed of 30 rats,and 24 rats in the DN group were successfully modeled.Two rats in DN and DN+emodin groups died during the experiment.(1)Body weight of rats at the end of the 14th week in control,DN,and DN+emodin groups was(350.1±14.16),(265.2±7.62)and(312.1±11.77)g,respectively,with statistically significant differences(F=136.50,P<0.001).(2)The respective renal SCr,BUN,BG,and 24-hour urine protein were(28.88±4.36)μmol/L,(8.76±0.50)mmol/L,(9.81±0.86)mmo

关 键 词：糖尿病肾病 大黄素 足细胞 上皮间质转化 疾病模型 动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]