不同pH微环境对牙本质修复相关基因表达影响的研究  

The expression of dentin restoration-related genes regulated by pH microenvironment.

作　　者：董少杰[1,2,3] 梅钰坤 张子琦 石昊羽 赵舒扬 牛林 谷冬华[1,2,4] DONG Shao-jie;MEI Yu-kun;ZHANG Zi-qi;SHI Hao-yu;ZHAO Shu-yang;NIU Lin;GU Dong-hua(Key laboratory of Shaanxi Province for Craniofacial Precision Medicine Research,College of Stomatology,Xi’an Jiaotong University,Shaanxi Xi’an 710004,China;Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases,Shaanxi Xi’an 710004,China;Department of Prosthodontics,College of Stomatology,Xi’an Jiaotong University,Shaanxi Xi’an 710004,China;Department of Special Clinic,College of Stomatology,Xi’an Jiaotong University,Shaanxi Xi’an 710004,China.)

机构地区：[1]西安交通大学口腔医院,陕西省颅颌面精准医学研究重点实验室,陕西西安710004 [2]陕西省牙颌疾病临床研究中心,陕西西安710004 [3]西安交通大学口腔医院口腔修复科,陕西西安710004 [4]西安交通大学口腔医院口腔特诊特需科,陕西西安710004

出　　处：《临床口腔医学杂志》2022年第5期259-263,共5页Journal of Clinical Stomatology

基　　金：国家自然科学基金面上项目(81970981);国家自然科学基金青年项目(82102221);西安交通大学基本科研业务费自由探索与创新-教师类项目(xzy012021069);西安市创新能力强基计划-医学研究项目(21YXYJ0123)。

摘　　要：目的:探讨成牙本质细胞在不同pH微环境下牙本质修复相关基因的表达情况。方法:以永生化成牙本质细胞系小鼠牙乳头细胞-23(murine dental papilla cells-23,MDPC-23)为研究对象,同时引入牙髓干细胞(dental pulp stem cells,DPSCs)作为对照,检测不同pH环境下牙本质修复相关基因在MDPC-23和DPSCs的表达情况。结果:MDPC-23细胞碱性磷酸酶(alkaline phosphatase,ALP)基因表达量在pH 6.5酸性环境表达上调,牙本质基质蛋白1(dentin matrix protein 1,DMP1)基因表达量在pH 6.5酸性环境培养6 h时高于对照组,I型胶原(collagen I,COL1)基因在pH 6.5酸性环境表达量上调,Runt相关转录因子2(Runt-related transcription factor 2,RUNX2)基因在pH 6.5酸性环境下培养6、12 h时表达上调,牙本质涎磷蛋白(dentin sialophospho protein,DSPP)基因的表达量在pH 8.5碱性环境表达上调。DPSCs细胞在酸性环境下相关基因的表达略有增加,与MDPC-23细胞存在差异。结论:MDPC-23细胞矿化相关基因在转录水平的表达受到微环境pH调控,并呈现出不同的表达特点。Objective:To explore the expression of dentin restoration-related genes in different pH microenvironments.Methods:The murine dental papilla cells MDPC-23 were taken as studying subject,taking DPSCs as the control.Realtime PCR were used to study the expression of dentin restoration-related genes of MDPC-23 cells under different pH conditions.Results:The expression of alkaline phosphatase(ALP)increased at pH 6.5,and the expression of dentin matrix protein 1(DMP1)was higher than that of the control group culturing at pH 6.5 for 6 h.The expression of collagen I(COL1)was upregulated after culturing at pH 6.5,and the expression of Runt-related transcription factor 2(RUNX2)gene was up-regulated after culturing at pH 6.5 for 6 h and 12 h.The expression of dentin sialophospho protein(DSPP)gene was up-regulated after culturing at pH 8.5.The expression of related genes in DPSCs was slightly upregulated in acid environment,which was different from MDPC-23.Conclusion:The expression of mineralization related genes in MDPC-23 differed at the transcriptional level under different pH conditions.

关 键 词：pH微环境 成牙本质细胞 牙髓干细胞 修复性牙本质 

分 类 号：Q813.1[生物学—生物工程]