珠海市男男性行为人群HIV-1感染者中HPgV-1基因特征分析及其基因型差异对艾滋病病程影响研究  

Genetic characteristics of human pegivirus-1(HPgV-1)in HIV-1-infected MSM in Zhuhai and the influence of HPgV-1 genotypes on the course of AIDS

作　　者：林毅雄 董莉[1] 黄文燕[1] 魏泉德[1] 黄辉涛 黄利群[1] Lin Yixiong;Dong li;Huang Wenyan;Wei Quande;Huang Huitao;Huang Liqun(Institute of Microbiology Inspection,Zhuhai Center for Disease Control and Prevention,Zhuhai 519000,China)

机构地区：[1]珠海市疾病预防控制中心微生物检验所,珠海519000

出　　处：《中华微生物学和免疫学杂志》2022年第6期428-433,共6页Chinese Journal of Microbiology and Immunology

基　　金：珠海市科技计划医疗卫生项目(ZH2201200004HJL,ZH22036201210188PWC)。

摘　　要：目的分析珠海市男男性行为(MSM)人群HIV-1感染者中人pegivirus-1(HPgV-1)流行及基因亚型分布情况,研究临床指标,揭示HPgV-1对艾滋病病程影响。方法采集珠海市2012—2020年MSM人群HIV-1抗体阳性血清标本934份,提取病毒RNA,采用套氏PCR方法扩增HPgV-15′UTR,阳性标本再扩增E基因并测序,通过系统进化树获得基因亚型传播情况;通过统计分析病例在艾滋病早期及未接受抗病毒治疗时的病毒载量及CD4+细胞数,分析HPgV-1感染对艾滋病病程影响。结果珠海市MSM人群HIV-1感染者中HPgV-1阳性率为31.05%,获得有效序列273份,G3型252例(占92.31%)占主要地位,与中国、日本等地近年流行株进化关系上高度同源;其次是G2型21例(占7.69%),与法国及美国等地流行株高度同源,本研究未发现G1、G4、G5、G6、G7基因亚型病例。通过对临床指标进行数据分析发现:HPgV-1 G3、G2基因型合并HIV-1感染与HIV-1单独感染,在HIV-1病毒载量和CD4+细胞数上差异无统计学意义,数据分散情况差异也无统计学意义。结论艾滋病早期及未接受抗病毒治疗MSM人群HIV-1感染者中,HPgV-1的感染未能延缓艾滋病病程,在中国广泛流行的HPgV-1 G3型并不能对艾滋病病程产生显著的积极影响,因此若需判断不同基因亚型HPgV-1与HIV-1相互作用机制,还需跟踪大量其他基因亚型HPgV-1病例进行系统深入研究,加强对HPgV-1致病机制,特别是该病毒与HIV在宿主体内相互作用的机制研究。Objective To analyze the prevalence and genotypes of human pegivirus-1(HPgV-1)among HIV-1-infected men who have sex with men(MSM)in Zhuhai,aiming to elucidate the impact of HPgV-1 on the progression of AIDS.Methods This study collected 934 serum specimens positive for antibodies against HIV-1 for viral RNA extraction from MSM in Zhuhai from 2012 to 2020.HPgV-15′UTR was amplified by nested PCR and then E gene was amplified by nested PCR and sequenced in the 5′UTR-positive specimens.A phylogenetic tree was constructed to analyze genotype distribution.The influence of HPgV-1 infection on the progression of AIDS was evaluated through analyzing HIV-1 viral load and CD4+cell counts in patients in the early stage of AIDS before antiviral treatment.Results The positive rate of HPgV-1 in MSM with HIV-1 infection in Zhuhai was 31.05%.A total of 273 valid sequences were obtained after amplification.The main genotype of HPgV-1 was G3(252,92.31%),which was highly homologous to the epidemic strains in China and Japan in recent years,followed G2(21,7.69%),which was highly homologous to the epidemic strains in France and America.HPgV-1 strains of G1,G4,G5,G6 and G7 genotypes were not detected.There was no significant difference in HIV-1 virus load or CD4+cell counts between patients with HIV-1 infection alone and those with HIV-1 and HPgV-1(G3 or G2 genotype)co-infection.Conclusions According to the data of this study,HPgV-1 infection could not delay the progression of AIDS in MSM in the early stage of AIDS before antiviral therapy.The widespread HPgV-1 of G3 genotype in China did not have a significant impact on the progression of AIDS.Therefore,a systematic in-depth research on various genotypes of HPgV-1 and further study on the pathogenic mechanism of HPgV-1,especially in patients with HPgV-1 and HIV co-infection,were needed to understanding the interaction mechanism between different genotypes of HPgV-1 and HIV-1.

关 键 词：人pegivirus-1 HIV-1 男男性行为 基因型 

分 类 号：R512.91[医药卫生—内科学]