A novel strategy for high-specificity, high-sensitivity, and high-throughput study for gut microbiome metabolism of aromatic carboxylic acids  

作　　者：Ningning Zhao Zhiqiang Liu Junpeng Xing Zhong Zheng Fengrui Song Shu Liu 

机构地区：[1]National Center of Mass Spectrometry in Changchun and Jilin Provincial Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China [2]Institute of Applied Chemistry and Engineering,University of Science and Technology of China,Hefei 230029,China [3]State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China

出　　处：《Chinese Chemical Letters》2022年第6期3031-3034,共4页中国化学快报（英文版）

基　　金：the National Natural Science Foundation of China (Nos. 82073973, 81872969);the Jilin Provincial Industrial Innovation Special Fund Project (No. 20200703015ZP);the Science and the Youth Innovation Promotion Association of CAS (No. 2019227)。

摘　　要：Aromatic carboxylic acids(ACAs) may be as transformed key metabolites via gut microbiome for playing better pharmacological effects. However, it's rare to achieve high-specificity, high-sensitivity, and highthroughput detection simultaneously, especially, for tracing trace ACAs in gut microbiome. In this work,firstly, a novel dual-template and double-shelled molecularly imprinted 96-well microplates(DDMIPs)was designed and amplified signal for p-hydroxybenzoic acid(PBA) and 3,4,5-trimethoxycinnamic acid(TMA). Additionally, the DDMIPs and a stable isotope labeling derivatization(SILD) method combined with the ultra-high performance liquid chromatography triple quadrupole tandem mass spectrometry(UHPLC-TQ MS) was firstly stepwise integrated, achieving high-effective, high-sensitive, and highthroughput study of gut microbiome metabolism. The whole strategy showed lower limits of detections(LODs) up to 1000 folds than the traditional method, and revealed a more real metabolism-time profile of PBA and TMA by 3-step signal amplification. The platform also laid the foundation for fast, simple,high-selective, high-effective, and high-throughput metabolism and pharmacological research.

关 键 词：Aromatic carboxylic acids Gut microbiome Dual-template and double-shelled molecularly imprinted 96-well microplate Stable isotope labeling derivatization UHPLC-TQ MS 

分 类 号：R285[医药卫生—中药学]