Exploiting styrene-maleic acid copolymer grafting chromatographic stationary phase materials for separation of membrane lipids  

作　　者：Mengying Liang Delu Liu Yangyang Nie Yanli Liu Xiaoqiang Qiao 

机构地区：[1]College of Pharmaceutical Sciences,Hebei Province Key Laboratory of Analytical Science&Technology,Key Laboratory of Medicinal Chemistry and Molecular Diagnosis,Ministry of Education,Hebei University,Baoding 071002,China

出　　处：《Chinese Chemical Letters》2022年第6期3123-3126,共4页中国化学快报（英文版）

基　　金：the financial support from National Natural Science Foundation of China(No.21675039);Natural Science Foundation of Hebei Province(Nos.B2019201327 and H2019201170);Hundred Outstanding Innovative Talents in Universities of Hebei Province(No.SLRC2019016);Young Talent of Hebei Province,Natural Science Interdisciplinary Research Program of Hebei University(No.DXK201912)。

摘　　要：High performance liquid chromatography-mass spectrometry is one of the most commonly used strategies for lipid analysis.The development of versatile chromatographic stationary phases to meet the increasing demands for separation of complex lipids is very important.Styrene-maleic acid(SMA)copolymer is an amphiphilic polymer,which has been proven to have the ability to solubilize lipid molecules of various structures.In this study,styrene-maleic anhydride copolymer coated silica was first prepared by the thiol-ene click reaction.With L-cysteine hydrochloride or dodecanol as the post-modification reagents,Sil-SMA-amino acid and Sil-SMA-dodecanol stationary phase materials were further successfully fabricated via nucleophilic ring-opening reaction.The Fourier-transform infrared,thermogravimetric analysis,and elemental analysis results confirmed the two stationary phase materials were successfully prepared.Furthermore,both the Sil-SMA-dodecanol column and the Sil-SMA-amino acid column possessed reversed-phase/hydrophilic interaction/ion exchange mixed-mode retention mechanisms.The column efficiency of the Sil-SMA-derivatives columns reached 77,300 N/m.Based on the mixed-mode retention characteristics,the Sil-SMA-derivatives columns achieved both the lipid classes and species separation via a single column.The Sil-SMA-amino acid column was further successfully used to separate lipid extract from gastric cancer cell membrane.All these results demonstrated that the SMA-based stationary phase materials have a good potential for use in lipid separation.

关 键 词：Styrene-maleic acid copolymer MIXED-MODE Stationary phase Chromatographic separation LIPIDS 

分 类 号：O657.63[理学—分析化学] TB383.2[理学—化学]