基于STR位点对广东省柑橘溃疡病菌种群遗传结构的分析  

Population Genetic Structure of Xanthomonas citri pv.citri in Guangdong Province Based on the STR Locus

作　　者：李文婷 李翠晓 林小清 郑永钦 郑正 邓晓玲[1] LI Wenting;LI Cuixiao;LIN Xiaoqing;ZHENG Yongqin;ZHENG Zheng;DENG Xiaoling(College of Plant Protection,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]华南农业大学植物保护学院,广州510642

出　　处：《园艺学报》2022年第6期1233-1246,共14页Acta Horticulturae Sinica

基　　金：广东省重点领域研发计划项目(2019B020217003)。

摘　　要：柑橘溃疡病是柑橘生产上最严重的病害之一,由柑橘黄单胞杆菌柑橘亚种(Xanthomonas citri pv.citri,Xcc)引起。为了全面系统地研究广东省柑橘溃疡病菌的种群遗传结构,通过比对分析已报道的中国8个柑橘溃疡病菌全基因组序列的串联重复位点(Short Tandem Repeat,STR),筛选出6个高度变异位点,对广东省的15个市(县)14个品种共352个柑橘溃疡病菌菌株进行遗传多样性分析。结果表明,基于遗传多样性和遗传距离对广东省不同地区的柑橘溃疡病菌株鉴定出6组类群:潮州和汕尾为Ⅰ组,梅州和河源为Ⅱ组,肇庆、广州、江门、佛山和惠州为Ⅲ组,阳江、茂名和湛江为Ⅳ组,云浮为Ⅴ组,韶关和清远为Ⅵ组。基于遗传多样性和遗传距离对广东省不同品种的柑橘溃疡病菌株鉴定出5组类群:沃柑、贡柑、朱砂橘、砂糖橘、茶枝柑、红江橙、马水橘为Ⅰ组,脐橙和柠檬为Ⅱ组,蕉柑、茂谷柑和春甜橘为Ⅲ组,沙田柚为Ⅳ组。Citrus canker is one of the most serious diseases in citrus production,which is caused by Xanthomonas citri pv.citri(Xcc).In order to systematically and comprehensively learn the genetic structure of citrus canker strains in Guangdong Province,six highly variable locus were selected by comparing and analyzing the short tandem repeat loci of the whole genome sequence of eight Chinese strains,and the genetic diversity of 352 strains of 14 varieties from 15 cities(counties)in Guangdong Province was analyzed.The results showed that based on the genetic diversity and genetic distance of Guangdong Province in different parts of the citrus canker strains identified six groups:Ⅰgroup(Chaozhou and Shanwei),Ⅱgroup(Meizhou and Heyuan),Ⅲgroup(Zhaoqing,Guangzhou,Jiangmen,Foshan and Huizhou),Ⅳgroup(Yangjiang,Maoming and Zhanjiang)Ⅴgroup(Yunfu),Ⅵgroup(Shaoguan and Qingyuan).Based on the genetic diversity and genetic distance on the different varieties of citrus canker in Guangdong strains identified five groups:Ⅰgroup(Wogan,Gonggan,Zhushaju,Shatamhju,Chazhigan,Hongjiangcheng and Mashuiju),Ⅱgroup(Qicheng and Ningmeng),Ⅲgroup(Jiaogan,Maogugan and Chuntianju),Ⅳgroup(Shatianyou).The results can provide a theoretical basis for the epidemic regularity of citrus canker disease in Guangdong province.

关 键 词：柑橘 柑橘溃疡病菌 短串联重复位点 遗传多样性 

分 类 号：S666[农业科学—果树学]