桃漆酶家族基因鉴定及PpLAC21功能分析  

Identification of Peach Laccase Family Genes and Function Analysis of PpLAC21

作　　者：邱可立 王玉民 何金铃[2] 俞红 潘海发[1] 盛玉[1] 谢庆梅 陈红莉[1] 周晖 张金云[1] QIU Keli;WANG Yumin;HE Jinling;YU Hong;PAN Haifa;SHENG Yu;XIE Qingmei;CHEN Hongli;ZHOU Hui;ZHANG Jinyun(Key Laboratory of Anhui Genetic Improvement and Ecophysiology of Horticultural Crop,Instisute of Horticulture,Anhui Academy of Agricultural Sciences,Hefei 230031,China;School of Life Science,Anhui Agricultural University,Hefei 230036,China;School of Biological and Food Engineering,Bozhou University,Bozhou,Anhui 236800,China;School of Forestry and Landscape Architecture,Anhui Agricultural University,Hefei 230036,China)

机构地区：[1]安徽省农业科学院园艺研究所,园艺作物种质创制及生理生态安徽省重点实验室,合肥230031 [2]安徽农业大学生命科学学院,合肥230036 [3]亳州学院生物与食品工程系,安徽亳州236800 [4]安徽农业大学林学与园林学院,合肥230036

出　　处：《园艺学报》2022年第6期1351-1362,共12页Acta Horticulturae Sinica

基　　金：国家现代农业产业技术体系建设专项资金项目(CARS-30-Z-05);安徽省果树产业技术体系项目(AHNYCYTX-10);安徽省重点研究和开发计划项目(202104a06020023,201904a06020047)。

摘　　要：利用生物信息技术鉴定了桃中漆酶(LAC)家族成员,分析其进化关系分析、基因结构、启动子区顺式作用元件以及表达模式。结果表明,在桃基因组中共鉴定出48个漆酶基因,通过在桃子叶愈伤组织中的表达模式分析,发现1个表达量很高的关键成员PpLAC21,在该基因沉默的桃愈伤组织中,木质素的含量下降,推测该基因可能参与桃愈伤组织的木质素合成。The members of laccase(LAC)family in peach[Prunus persica L.(Batsch)]were identified by bioinformatics technology,and their evolutionary relationship,gene structure,cis-elements in promoter region and expression pattern were analyzed.The results showed that a total of 48 laccase genes were identified in peach genome.Through the analysis of expression pattern in peach cotyledon callus,it was found that a key member with high expression,PpLAC21,decreased the content of lignin in peach callus silenced by this gene.It is speculated that this gene may be involved in lignin synthesis in peach callus.

关 键 词：桃 漆酶 愈伤组织 病毒介导的基因沉默(VIGS) 

分 类 号：S662.1[农业科学—果树学]