Warm temperature compromises JA-regulated basal resistance to enhance Magnaporthe oryzae infection in rice  

作　　者：Jiehua Qiu Junhui Xie Ya Chen Zhenan Shen Huanbin Shi Naweed I.Naqvi Qian Qian Yan Liang Yanjun Kou 

机构地区：[1]State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 311400,China [2]Temasek Life Sciences Laboratory,and Department of Biological Sciences,1 Research Link,National University of Singapore,Singapore 117604,Singapore [3]College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China

出　　处：《Molecular Plant》2022年第4期723-739,共17页分子植物（英文版）

基　　金：supported in part by the National Natural Science Foundation of China(32000103 to Y.K.);Zhejiang Provincial Natural Science Foundation of China(LQ19C130007);key R&D project of China National Rice Research Institute,grand number“CNRRI-2020-04”;supported by the Chinese Academy of Agricultural Sciences under the“Elite Youth”Program and the Agricultural Science and Technology Innovation Program.N.I.N acknowledges funding support from the Temasek Life Sciences Laboratory,Singapore.

摘　　要：Changes in global temperatures profoundly affect the occurrence of plant diseases.It is well known that rice blast can easily become epidemic in relatively warm weather.However,the molecular mechanism remains unclear.In this study,we show that enhanced blast development at a warm temperature(22C)compared with the normal growth temperature(28C)is rice plant-determined.Comparative transcriptome analysis revealed that jasmonic acid(JA)biosynthesis and signaling genes in rice could be effectively induced by Magnaporthe oryzae at 28C but not at 22C.Phenotypic analyses of the osaoc1 and osmyc2 mutants,OsCOI1 RNAi lines,and OsMYC2-OE plants further demonstrated that compromised M.oryzaeinduced JA biosynthesis and signaling lead to enhanced blast susceptibility at the warm temperature.Consistent with these results,we found that exogenous application of methyl jasmonate served as an effective strategy for improving blast resistance under the warm environmental conditions.Furthermore,decreased activation of JA signaling resulted in the downregulated expression of some key basal resistance genes at 22C when compared with 28C.Among these affected genes,OsCEBiP(chitin elicitorbinding protein precursor)was found to be directly regulated by OsMYB22 and its interacting protein OsMYC2,a key component of JA signaling,and this contributed to temperature-modulated blast resistance.Taken together,these results suggest that warm temperature compromises basal resistance in rice and enhances M.oryzae infection by reducing JA biosynthesis and signaling,providing potential new strategies for managing rice blast disease under warm climate conditions.

关 键 词：temperature-modulated resistance rice blast jasmonic acid CEBiP MYB22 plant immunity 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]