A well-supported nuclear phylogeny of Poaceae and implications for the evolution of C4 photosynthesis  

作　　者：Weichen Huang Lin Zhang JTravis Columbus Yi Hu Yiyong Zhao Lin Tang Zhenhua Guo Wenli Chen Michael McKain Madelaine Bartlett Chien-Hsun Huang De-Zhu Li Song Ge Hong Ma 

机构地区：[1]Department of Biology,510 Mueller Laboratory,Huck Institutes of the Life Sciences,The Pennsylvania State University,University Park,State College,PA 16802,USA [2]Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering and State Key Laboratory of Genetic Engineering,Institute of Biodiversity Sciences and Institute of Plant Biology,School of Life Sciences,Fudan University,2005 Songhu Road,Shanghai 200438,China [3]Rancho Santa Ana Botanic Garden and Claremont Graduate University,1500 North College Avenue,Claremont,CA 91711,USA [4]College of Life Sciences,Zhejiang University,Hangzhou,Zhejiang 310058,China [5]Plant Germplasm and Genomics Center,Germplasm Bank of Wild Species,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming,Yunnan 650201 China [6]State Key Laboratory of Systematic and Evolutionary Botany,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China [7]Department of Biological Sciences,University of Alabama,411 Mary Harmon Bryant Hall,Tuscaloosa,AL 35487,USA [8]Biology Department,University of Massachusetts Amherst,611 North Pleasant Street,221 Morrill 3,Amherst,MA 01003 USA

出　　处：《Molecular Plant》2022年第4期755-777,共23页分子植物（英文版）

基　　金：supported by funds from Eberly College of Science and the Huck Institutes of the Life Sciences at the Pennsylvania State University and from grants from the National Natural Science Foundation of China(31770242 and 31970224);funds from the Ministry of Education Key Laboratory of Biodiversity Science and Ecological Engineering and State Key Laboratory of Genetic Engineering at Fudan University.

摘　　要：Poaceae(the grasses)includes rice,maize,wheat,and other crops,and is the most economically important angiosperm family.Poaceae is also one of the largest plant families,consisting of over 11000 species with a global distribution that contributes to diverse ecosystems.Poaceae species are classified into 12 subfamilies,with generally strong phylogenetic support for their monophyly.However,many relationships within subfamilies,among tribes and/or subtribes,remain uncertain.To better resolve the Poaceae phylogeny,we generated 342 transcriptomic and seven genomic datasets;these were combined with other genomic and transcriptomic datasets to provide sequences for 357 Poaceae species in 231 genera,representing 45 tribes and all 12 subfamilies.Over 1200 low-copy nuclear genes were retrieved from these datasets,with several subsets obtained using additional criteria,and used for coalescent analyses to reconstruct a Poaceae phylogeny.Our results strongly support the monophyly of 11 subfamilies;however,the subfamily Puelioideae was separated into two non-sister clades,one for each of the two previously defined tribes,supporting a hypothesis that places each tribe in a separate subfamily.Molecular clock analyses estimated the crown age of Poaceae to be101 million years old.Ancestral character reconstruction of C3/C4 photosynthesis supports the hypothesis of multiple independent origins of C4 photosynthesis.These origins are further supported by phylogenetic analysis of the ppc gene family that encodes the phosphoenolpyruvate carboxylase,which suggests that members of three paralogous subclades(ppc-aL1a,ppc-aL1b,and ppcB2)were recruited as functional C4 ppc genes.This study provides valuable resources and a robust phylogenetic framework for evolutionary analyses of the grass family.

关 键 词：GRAMINEAE transcriptome nuclear phylogeny molecular clock C4 photosynthesis ppc gene evolution 

分 类 号：S51[农业科学—作物学] Q943.2[生物学—植物学]