Supramolecular assembly of chloroplast NADH dehydrogenase-like complex with photosystem Ⅰfrom Arabidopsis thaliana  

作　　者：Xiaodong Su Duanfang Cao Xiaowei Pan Lifang Shi Zhenfeng Liu Luca Dall’Osto Roberto Bassi Xinzheng Zhang Mei Li 

机构地区：[1]National Laboratory of Biomacromolecules,CAS Center for Excellence in Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,P.R.China [2]University of Chinese Academy of Sciences,Beijing 100049,P.R.China [3]College of Life Science,Capital Normal University,Beijing 100101,P.R.China [4]Dipartimento di Biotecnologie,Universitàdi Verona,37134 Verona,Italy [5]Center for Biological Imaging,CAS Center for Excellence in Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,P.R.China

出　　处：《Molecular Plant》2022年第3期454-467,共14页分子植物（英文版）

基　　金：The project was funded by the Strategic Priority Research Program of the CAS(XDB27020106);the National Natural Science Foundation of China(31930064,31970264,31770778);the CAS Project for Young Scientists in Basic Research(#YSBR-015);the National Key R&D Program of China(2017YFA0503702);R.B.and L.D.thank the University of Verona(grant RIBA 2017 to R.B.);MUR(grant PRIN 2018 to L.D.)for financial support.X.S.,D.C.and X.P.are sponsored by the Youth Innovation Promotion Association at the Chinese Academy of Sciences(2018123,2018124 and 2018128).

摘　　要：Cyclic electron transport/flow(CET/CEF)in chloroplasts is a regulatory process essential for the optimization of plant photosynthetic efficiency.A crucial CEF pathway is catalyzed by a membrane-embedded NADH dehydrogenase-like(NDH)complex that contains at least 29 protein subunits and associates with photosystem I(PSI)to form the NDH-PSI supercomplex.Here,we report the 3.9Åresolution structure of the Arabidopsis thaliana NDH-PSI(AtNDH-PSI)supercomplex.We constructed structural models for 26 AtNDH subunits,among which 11 are unique to chloroplasts and stabilize the core part of the NDH complex.In the supercomplex,one NDH can bind up to two PSI-light-harvesting complex I(PSI-LHCI)complexes at both sides of its membrane arm.Two minor LHCIs,Lhca5 and Lhca6,each present in one PSI-LHCI,interact with NDH and contribute to supercomplex formation and stabilization.Collectively,our study reveals the structural details of the AtNDH-PSI supercomplex assembly and provides a molecular basis for further investigation of the regulatory mechanism of CEF in plants.

关 键 词：cyclic electron transport/flow NADH dehydrogenase-like(NDH)complex photosystem I structure Arabidopsis thaliana 

分 类 号：Q94[生物学—植物学]