Effects of ginger-partitioned moxibustion on the expression levels of PGF2α, E2, P, and mRNAs of PGF2αR and E2R in rats with primary dysmenorrhea due to cold-dampness stagnation  

隔姜灸对寒湿凝滞型原发性痛经大鼠PGF2α、E2、P、PGF2αR及E2R mRNAs表达的影响

作　　者：LIU Chao LI Xinhua ZHOU Chenxi LIANG Yulei ZHANG Xuanping LIU Yucai ZHAO Zhiguo MA Xiaoshun 刘超;李新华;周晨曦;梁玉磊;张选平;刘昱材;赵志国;马小顺(Hebei University of Chinese Medicine,Shijiazhuang,050200,China;Key Research Office of Hebei Province Needling and Moxibustion Techniques Effect Specificity,Shijiazhuang,050200,China)

机构地区：[1]Hebei University of Chinese Medicine,Shijiazhuang,050200,China [2]Key Research Office of Hebei Province Needling and Moxibustion Techniques Effect Specificity,Shijiazhuang,050200,China

出　　处：《Journal of Acupuncture and Tuina Science》2022年第2期104-110,共7页针灸推拿医学（英文版）

基　　金：河北省高等学校科学技术研究项目,No.ZD2019099。

摘　　要：Objective To observe the effects of ginger-partitioned moxibustion at Shenque(CV8)and Guanyuan(CV4)on the expression levels of endocrine-related molecules and their receptors in rats with primary dysmenorrhea(PD)due to cold-dampness stagnation,thus to explore their analgesic mechanisms.Methods Thirty-two female Wistar rats were divided into a normal group,a model group,a ginger-partitioned moxibustion group,and a Western medicine group according to the random number table method,with 8 rats in each group.Except for rats in the normal group,all other rats were treated with oxytocin combined with ice-water bath to establish the rat models of PD due to cold-dampness stagnation.After successful modeling,rats in the normal group and the model group did not receive treatment;rats in the ginger-partitioned moxibustion group received treatments with ginger-partitioned moxibustion at Shenque(CV8)and Guanyuan(CV4);rats in the Western medicine group received ibuprofen by intragastric administration.The writhing response of rats was compared among groups,and the serum levels of prostaglandin F2α(PGF2α),estrogen(estradiol,E2),progesterone(P),and the mRNA expression of PGF2αand E2 receptors in the uterine tissues were detected.Results No writhing behavior was observed in the normal group;compared with the normal group,the serum PGF2αand E2 levels in the model group were increased(P<0.01),while the P level was decreased(P<0.01),and the mRNA expression levels of the uterine PGF2αand E2 receptors were increased(P<0.01,P<0.05).Compared with the model group,the writhing behavior latency was prolonged,and the writhing response score was decreased in the ginger-partitioned moxibustion group and the Western medicine group(P<0.01);the serum PGF2αand E2 levels in the ginger-partitioned moxibustion group and the Western medicine group were decreased,while the P level was increased(P<0.05 or P<0.01);the mRNA expression levels of the uterine PGF2αand E2 receptors in the ginger-partitioned moxibustion group and the Western medicine g目的观察隔姜灸神阙、关元对类寒湿凝滞型原发性痛经(PD)大鼠内分泌相关分子及其受体基因表达的影响,探讨其镇痛机制。方法将32只雌性Wistar大鼠根据随机数字表法分为正常组、模型组、隔姜灸组和西药组,每组8只。除正常组外,其余各组使用缩宫素联合冰水浴法建立类寒湿凝滞型PD大鼠模型。造模成功后,正常组和模型组不接受治疗;隔姜灸组接受隔姜灸神阙和关元治疗;西药组接受布洛芬灌胃治疗。比较各组大鼠扭体反应情况,检测血清中前列腺素F2α(PGF2α)、雌激素(E2)、孕激素(P)含量及子宫组织中PGF2α受体mRNA、E2受体mRNA表达。结果正常组未出现扭体行为;与正常组比较,模型组血清PGF2α、E2含量增加(P<0.01),P含量下降(P<0.01);子宫PGF2α受体mRNA、E2受体mRNA表达增加(P<0.01,P<0.05)。与模型组比较,隔姜灸组、西药组扭体行为潜伏期延长,扭体反应评分降低(P<0.01);隔姜灸组、西药组血清PGF2α、E2含量下降,P含量上升(P<0.05 or P<0.01);隔姜灸组、西药组子宫PGF2α受体mRNA、E2受体mRNA表达减少(P<0.05)。与西药组比较,隔姜灸组扭体行为潜伏期延长,扭体反应评分降低(P<0.05),血清E2含量下降(P<0.05),而血清PGF2α和P含量及子宫PGF2α受体mRNA、E2受体mRNA表达无统计学差异(P<0.05)。结论隔姜灸对类寒湿凝滞型PD的镇痛效应可能与调控子宫组织中PGF2α受体mRNA及E2受体mRNA表达水平有关。

关 键 词：Moxibustion Therapy Indirect Moxibustion Ginger-partitioned Moxibustion DINOPROST Estradiol DYSMENORRHEA RATS 

分 类 号：R2-03[医药卫生—中医学]