点蜂缘蝽铁蛋白基因功能分析  

Functional analysis of ferritin genes in Riptortus pedestris

作　　者：闫箫天 李俊敏 孙宗涛 张传溪 陈剑平 黄海剑 YAN Xiao-Tian;LI Jun-Min;SUN Zong-Tao;ZHANG Chuan-Xi;CHEN Jian-Ping;HUANG Hai-Jian(Institute of Plant Virology,Ningbo University,Ningbo 315211,China)

机构地区：[1]宁波大学植物病毒学研究所,宁波315211

出　　处：《应用昆虫学报》2022年第3期568-577,共10页Chinese Journal of Applied Entomology

基　　金：宁波市公益类科技计划(2021S012);转基因生物新品种培育重大专项(2019ZX08004-004)。

摘　　要：【目的】铁蛋白(Ferritin)在维持生物铁稳态中起着重要作用。通过分析点蜂缘蝽Riptortus pedestris铁蛋白基因,揭示其对点蜂缘蝽的生物学影响,为进一步探究铁蛋白功能以及点蜂缘蝽防治提供参考。【方法】利用转录组和基因组数据鉴定点蜂缘蝽铁蛋白基因;通过转录组测序分析铁蛋白基因在不同龄期和不同组织的表达水平;对点蜂缘蝽若虫分别注射铁蛋白基因dsRNA进行基因沉默。【结果】本研究鉴定到了3个点蜂缘蝽铁蛋白基因,分别是铁蛋白重链亚基(RpFer1)、铁蛋白轻链亚基(RpFer2)和体铁蛋白(Rpsoma-Fer)。3种铁蛋白基因在各个龄期均有表达,RpFer1和RpFer2的表达随昆虫蜕皮呈周期性变化,而Rpsoma-Fer的表达与昆虫蜕皮无明显相关性。组织特异性分析表明,3种铁蛋白在肠道、肌肉以及表皮组织中表达较高,而在唾液腺、精巢、卵巢的表达较低。RNA干扰结果表明,抑制RpFer1和RpFer2会导致点蜂缘蝽若虫蜕皮受阻,进而表现出高致死率,但抑制Rpsoma-Fer没有出现明显的表型变化。【结论】铁蛋白RpFer1和RpFer2在点蜂缘蝽的发育过程中不可或缺,可能在蜕皮过程中起重要作用,未来可以将其作为RNA干扰技术防治点蜂缘蝽的靶基因。[Objectives]To characterize the function of ferritin genes,which play an essential role in maintaining iron homeostasis,in Riptortus pedestri,thereby providing a theoretical foundation for the control of this pest.[Methods]Homologous ferritin genes were identified by sequence analysis from R.pedestris transcriptomic and genomic data and RNA-seq used to detect the expression patterns of three ferritin genes in different developmental stages and tissues.Third instar nymphs were injected with ferritin dsRNAs and subsequent phenotypic changes observed.[Results]Three ferritin genes,including ferritin heavy chain(RpFer1),ferritin light chain(RpFer2)and soma-Fer(Rpsoma-Fer),were identified.All three genes were expressed at all developmental stages examined,but differed in their expression patterns in different stages.The expression patterns of RpFer1 and RpFer2 were correlated with molting,whereas that of Rpsoma-Fer was not.All three genes were highly expressed in the intestine,muscle,and cuticle but less expressed in the salivary gland,testis,and ovary.Suppression of RpFer1 or RpFer2 was lethal to R.pedestris nymphs because it inhibited molting.However,we did not detect any phenotypic changes after treatment with dsRpsoma-Fer.[Conclusion]Both RpFer1 and RpFer2 play an essential role in the molting process and are therefore essential for normal R.pedestris development.Consequently these two genes are potential targets for R.pedestris control.

关 键 词：点蜂缘蝽 防治 铁蛋白 RNA干扰 

分 类 号：S433.3[农业科学—农业昆虫与害虫防治]