金纳米颗粒和多壁碳纳米管协同增敏的维生素C传感器的研制与应用  

Development and application of vitamin C sensor with synergistic sensitization of gold nanoparticles and multi walled carbon nanotubes

作　　者：栗云鹏 施玉峰 巫彬芳 周华 施震渺 李松浩 徐海涛 兰玉彬 龙拥兵 LI Yunpeng;SHI Yufeng;WU Binfang;ZHOU Hua;SHI Zhenmiao;LI Songhao;XU Haitao;LAN Yubin;LONG Yongbing(College of Electronic Engineering,South China Agricultural University/National Center for International Collaboration Research on Precision Agricultural Aviation Pesticides Spraying Technology,Guangzhou 510642,China)

机构地区：[1]华南农业大学电子工程学院/国家精准农业航空施药技术国际联合研究中心,广东广州510642

出　　处：《华南农业大学学报》2022年第5期115-123,共9页Journal of South China Agricultural University

基　　金：广东省重点领域研发计划(2019B020219002);国家自然科学基金(11774099);广东省自然科学基金(2021A1515012112,2022A1515010411);广东省高校特色创新项目(2019KTSCX018)。

摘　　要：【目的】开发一种低成本、高响应的维生素C电化学传感器,用于实现果蔬中维生素C含量的快速检测。【方法】通过在铅笔芯电极上修饰金纳米颗粒和多壁碳纳米管,构建具有维生素C强催化响应的MWCNTs/Au/PGE电极。通过扫描电镜、X射线光电子能谱、拉曼光谱和循环伏安法表征电极,采用差分脉冲伏安法确定电极的pH适用范围和最优pH条件。采用时间电流法建立标准曲线和方程,以实现快速检测。最后通过加标回收率法检测番茄中维生素C含量。【结果】基于MWCNTs/Au/PGE电极的维生素C电化学传感器可在pH 4~8范围内准确地测定维生素C含量,在pH 5时性能最优。快速检测时检测维生素C的质量分数范围为1~500μg/g,灵敏度达0.244μA·(μg/g)^(−1)·cm^(-2)。该传感器对葡萄糖、苹果酸和柠檬酸的干扰率均小于1.77%,同一传感器多次测定的相对标准偏差为2.7%。成功检出番茄样品中的维生素C质量分数为69.42μg/g,加标回收率为109%~113%,相对标准偏差小于2.26%。【结论】MWCNTs/Au/PGE电极制备工艺简单,成本低,灵敏度高,测量范围宽,有较强的稳定性和抗干扰性,为实现快速检测果蔬中维生素C提供了新思路。【Objective】A low-cost and high response electrochemical sensor for vitamin C was developed to realize the rapid detection of vitamin C content in fruits and vegetables.【Method】MWCNTs/Au/PGE electrode with strong catalytic response to vitamin C was constructed by modifying gold nanoparticles and multi walled carbon nanotubes on pencil lead electrode.The electrode was characterized by scanning electron microscopy,X-ray photoelectron spectroscopy,Raman spectroscopy and cyclic voltammetry.The application range and optimal condition of the electrode pH were determined by differential pulse voltammetry.The standard curve and equation were established by time-current method to realize rapid detection.Finally,the vitamin C content of tomato was detected by standard addition recovery method.【Result】The vitamin C electrochemical sensor based on MWCNTs/Au/PGE electrode could accurately determine the vitamin C content in the range of pH 4-8,and its performance was the best when pH was 5.During rapid detection,the detection mass fraction range was 1-500μg/g,sensitivity up to 0.244μA·(μg/g)^(−1)·cm^(-2).The interference rates of the sensor to glucose,malic acid and citric acid were less than 1.77%,and the relative standard deviation(RSD)measured by the same sensor for many times was 2.7%.The vitamin C content of tomato sample was 69.42μg/g,the recovery was 109%-113%,and the RSD was less than 2.26%.【Conclusion】MWCNTs/Au/PGE electrode has the advantages of simple preparation process,low cost,high sensitivity,wide measurement range,strong stability and anti-interference.It provides a new idea for the rapid detection of vitamin C in fruits and vegetables.

关 键 词：维生素C 多壁碳纳米管 电化学传感器 果蔬 

分 类 号：O657.1[理学—分析化学] S24[理学—化学]