基于55K SNP芯片检测小麦株高QTL  

Detection of QTLs for plant height in wheat based on the 55K SNP array

作　　者：廖思敏 冯波[1] 徐智斌[1] 樊小莉[1] 周强[1] 纪光思 刘小凤 余琴 王涛[1] LIAO Simin;FENG Bo;XU Zhibin;FAN Xiaoli;ZHOU Qiang;JI Guangsi;LIU Xiaofeng;YU Qin;WANG Tao(Chengdu Institute of Biology,Chinese Academy of Sciences,Chengdu 610041,China;University of Chinese Academy of Science,Beijing 100049,China)

机构地区：[1]中国科学院成都生物研究所,成都610041 [2]中国科学院大学,北京100049

出　　处：《应用与环境生物学报》2022年第3期576-581,共6页Chinese Journal of Applied and Environmental Biology

基　　金：中国科学院战略性先导科技专项“种子精准设计与创造”项目(XDA2403042)资助。

摘　　要：株高是小麦遗传改良的重要性状之一,为进一步挖掘小麦株高的数量性状位点(quantitative trait locus,QTL),以小麦品系W7268和小麦品种川育12杂交得到的包含180个家系的重组自交群体(RIL)为材料,利用55K SNP芯片构建高密度遗传图谱,根据3年共5个环境的数据对株高性状进行QTL定位分析.结果表明,该图谱包含1598个SNP标记,全长2363.54 cM,含有22个连锁群,覆盖全部21条染色体.A、B、D三个基因组分别含有428、703和467个标记;覆盖染色体长度分别为741.89、678.17和943.48 cM.对株高性状进行QTL分析,共检测出52个QTL,分布于1A、4A、5A、3B、4B、6B、7B、2D、3D、5D和7D染色体上,有25个QTL的加性效应来源于母本W7268,其余来自父本川育12.单个QTL表型解释变异率为1.34%-27.61%,其中稳定主效的QTL有两个,分别为QPh.cib-4B.1和QPh.cib-2D.1,其贡献率分别为6.43%-27.61%和4.90%-11.97%.亲本及群体的Ppd-D1标记检测及遗传效应分析结果表明,QPht.cib-2D.1可能为Ppd-D1,相较于Ppd-D1b,单倍型Ppd-D1a可降低株高0.77-5.55 cm(0.83%-5.86%).本研究建立的遗传图谱可用于有效识别和发掘优异的遗传位点,鉴定的小麦株高QTL可用于小麦株高的分子标记辅助育种.(图3表3参29)Plant height(Pht)is a crucial trait related to plant architecture and yield.The purpose of this study was to identify QTLs(quantitative trait loci)for Pht in wheat.A recombinant inbred line population comprising 180 lines derived from the cross between a wheat line‘W7268’and a wheat variety‘Chuanyu12’was genotyped using a Wheat 55K single-nucleotide polymorphism(SNP)array to construct a genetic map and to identify QTLs.A genetic map consisting of 22 linkage groups was constructed with 1598 SNP markers corresponding to 21 chromosomes of the wheat genome covering 2363.54 cM.The map length and locus number were unequally divided among the three sub-genomes with lengths of 741.89,678.17,and 943.48 cM and 428,703,and 467 loci for the A,B,and D genomes,respectively.A total of 52 Pht QTLs were detected in all five environments.A single QTL explained a phenotypic variance of 1.34%-27.61%,with 25 QTL additive effects from W7268,and the remaining from CY12.Two major and stable QTLs were detected on chromosomes 4B and 2D,which explained 6.43%to 27.61%and 4.90%to 11.97%of the phenotypic variation,respectively.QPht.cib-2D.1 is possibly Ppd–D1(photoperiod).The genotypes of Ppd-D1 in the parents and the population were detected,and their effect on height was estimated.Compared with Ppd-D1b,Ppd-D1a was associated with significant reductions of 0.77-5.55 cm(0.83%-5.86%)in height.The genetic map developed in this study can be used to efficiently excavate and identify excellent genetic loci,and QTLs for Pht can be used in molecular marker-assisted breeding.

关 键 词：普通小麦 株高 QTL 55K SNP芯片 

分 类 号：S512.1[农业科学—作物学]