小麦乙烯转录因子TaERF2响应湿害胁迫的表达分析  

Expression Analysis of Wheat Ethylene Responsive Factor TaERF2 Response to Waterlogging Stress

作　　者：宋桂成 周淼平[1] 余桂红[1] 姚金保[1] 马鸿翔 SONG Guicheng;ZHOU Miaoping;YU Guihong;YAO Jinbao;MA Hongxiang(Institute of Food Crops,Jiangsu Academy of Agricultural Sciences,Nanjing,Jiangsu 210014;College of Agronomy,Yangzhou University,Yangzhou,Jiangsu 225009)

机构地区：[1]江苏省农业科学院粮食作物研究所,江苏南京210014 [2]扬州大学农学院,江苏扬州225009

出　　处：《核农学报》2022年第5期876-884,共9页Journal of Nuclear Agricultural Sciences

基　　金：国家现代农业技术产业技术体系(CARS-03)。

摘　　要：乙烯响应因子在植物非生物胁迫应答中起重要作用。为了解TaERF2在小麦湿害胁迫下作用的分子机理,本研究对TaERF2基因及其编码蛋白序列特征进行分析,并检测TaERF2在不同耐湿品种中的表达特征及原核表达。结果表明,TaERF2基因含有2个外显子和1个内含子,编码355个氨基酸序列,具有典型的AP2/ERF保守结构域和YRG、RAYD基序。AP2/ERF家族转录因子进化和同源比对分析表明,TaERF2属于AP2/ERF家族B2组,与拟南芥AtRAP2.12、AtHRE1和水稻OsSNORKEL1、OsSNORKEL2有较高的同源性,且启动子区域含有缺氧厌氧顺式元件,推测TaERF2可能为湿害胁迫响应基因。湿害胁迫后,TaERF2在小麦根系中特异表达,在耐湿小麦品种宁麦9号根系中的表达显著上调,2~4 h表达量达到最高,然后显著降低,而在不耐湿小麦品种郑麦1354根系中的表达无显著上调。原核表达结果显示,TaERF2可以快速响应ZPTG刺激诱导,与上述宁麦9号根系中的表达模式相似,表明TaERF2在小麦耐湿中具有重要调节作用。本研究为解析小麦耐湿分子调控机制提供了理论参考。Ethylene response factors play an important role in plant abiotic stress response.In order to understand the molecular mechanism of TaERF2 under waterlogging in wheat,the sequence characteristics of TaERF2 gene and its coding protein were analyzed,and the expression characteristics and prokaryotic expression of TaERF2 were detected in different waterlogging tolerant cultivars.The results showed that TaERF2 gene contained two exons and one intron encoded 355 amino acid sequences which had typical AP2/ERF conserved domain and YRG and RAYD motifs.The evolution and homology comparison analysis of AP2/ERF family transcription factors showed that TaERF2 belonged to group B2 of AP2/ERF family,which had high homology with Arabidopsis AtRAP2.12,AtHRE1 and rice OsSNORKEL1 and OsSNORKEL2.The promoter region of TaERF2 contained anoxic and anaerobic cis elements.It was speculated that TaERF2 might be a response gene to wet stress.After waterlogging stress,TaERF2 was specifically expressed in wheat roots.The expression was significantly up-regulated in the roots of waterlogging tolerant cultivar Ningmai 9,which reached the highest expression in 2-4 h,and then decreased,but not up-regulated in the roots of waterlogging sensitive cultivar Zhengmai 1354.Prokaryotic expression showed that TaERF2 could respond quickly to stimulation induction,which was similar to the expression pattern of that in the root of Ningmai 9.The results suggested that TaERF2 plays an important regulatory role in wheat moisture tolerance,which provides reference for the study of AP2/ERF transcription factor in the molecular regulation mechanism of wheat waterlogging tolerance.

关 键 词：小麦 乙烯响应因子 湿害 启动子 原核表达 

分 类 号：S512.1[农业科学—作物学]