污泥脱水液培养小球藻体系中藻菌关系研究  

Study on the Algae-Bacteria Relationship in Chlorella vulgaris Cultured System with Sludge Dewatering Liquid

作　　者：庄海浪 任洪艳 朱文秀 朱国庆 Zhuang Hailang;Ren Hongyan;Zhu Wenxiu;Zhu Guoqing(Jiangsu Engineering Laboratory for Biomass Energy and Carbon Reduction Technology,School of Environmental and Civil Engineering,Jiangnan University,Wuxi,214122)

机构地区：[1]江南大学环境与土木工程学院,江苏省生物质能与减碳技术工程实验室,无锡214122

出　　处：《基因组学与应用生物学》2022年第1期60-71,共12页Genomics and Applied Biology

基　　金：国家自然科学基金青年科学基金项目(21808087)资助。

摘　　要：为探究污泥脱水液中自生菌对小球藻(Chlorella vulgaris)生长的影响及其相互关系,本研究设置了藻菌共培养(A+B)、小球藻单独培养(A)、细菌单独培养(原污泥脱水液,B)三组对比实验。经过72 h培养,藻菌共培养组中小球藻的生物量为1.59 g/L,显著高于小球藻单独培养组的1.24 g/L,表明藻菌共培养能促进小球藻的生长。培养过程中对CO_(2)和O_(2)的监测发现,小球藻与细菌之间能够相互利用彼此释放的CO_(2)和O_(2)实现互利共生。藻菌共培养组中COD、TP、TN、NH_(4)^(+)-N的利用率分别为84.8%、81.4%、75.5%、86.8%,均大于藻、菌单独培养组的,且小球藻对TP、TN、NH_(4)^(+)-N的利用率相对较大,自生菌与小球藻对COD的利用率相当。胞外聚合物的检测显示,小球藻分泌的蛋白质与多糖明显高于自生菌的。高通量测序表明,共培养体系中的优势菌属假单胞菌属(Pseudomonas,丰度为44.2%)、Alicycliphilus(丰度为25.7%)、Gemmobacter(丰度为13.5%)、黄杆菌属(Flavobacterium,丰度为6.9%)。扫描电子显微镜观察到藻菌之间通过胞外聚合物粘连在一起成簇团生长。In order to explore the influence of wastewater-borne bacteria of sludge dewatering liquid on the growth of Chlorella vulgaris and the interactions of algae-bacteria symbiotsis,three systems of co-cultivation of algae and bacteria(A+B),cultivation of algae separately(A),and cultivation of wastewater-borne bacteria separately(B)were set.The biomass of Chlorella vulgaris was 1.59 g/L in the system of co-cultivation,which was significantly higher than that in the system of cultivation of Chlorella vulgaris separately 1.24 g/L,after 72 hours of cultivation,indicating that the co-cultivation of algae-bacteria could promote the growth of Chlorella vulgaris.The monitoring of CO_(2)and O_(2)showed that Chlorella vulgaris and bacteria could utilize CO_(2)and O_(2)released each other,thus achieving mutualism.In the system of co-cultivation,the utilization rates of COD,TP,TN and NH_(4)^(+)-N were 84.8%,81.4%,75.5%and 86.8%,respectively,which were all higher than that in the systems of cultivation of algae and bacteria separately.In addition,TP、TN and NH_(4)^(+)-N could be removed much more by the Chlorella vulgaris than that by the bacteria,and the utilization rate of COD by wastewater-borne bacteria and Chlorella vulgaris was similar.The results of the extracellular polymers showed that the protein and polysaccharide secreted by Chlorella vulgaris were significantly higher than that of wastewater-borne bacteria.The high-throughput sequencing results showed that four genera of Pseudomonas(Abundance was 44.2%),Alicycliphilus(Abundance was 25.7%),Gemmobacter(Abundance was 13.5%)and Flavobacterium(Abundance was 6.9%)were the dominant genara and could be symbiotic with Chlorella vulgaris in the co-cultivation system.Scanning electron microscope observed algae and bacteria growing together in clusters through extracellular polymer adhesion.

关 键 词：小球藻 自生菌 污泥脱水液 共培养 藻菌关系 

分 类 号：X703[环境科学与工程—环境工程]