甘蔗磷效率的基因型差异及磷转运子Pht1的表达分析  

Genotypic Differences in Phosphorus Efficiency and Expression Analysis of Phosphorus Transporter Pht1 in Sugarcane(Saccharum officinarum)

作　　者：刘莹 易科[1] 杨曙 陈迪文 凌桂芝 唐新莲 黎晓峰[1] 赵尊康 Liu Ying;Yi Ke;Yang Shu;Chen Diwen;Ling Guizhi;Tang Xinlian;Li Xiaofeng;Zhao Zunkang(State Key Laboratory of Protection and Utilization of Subtropical Agricultural Biological Resources,Guangxi Key Laboratory of Sugarcane Biology,College of Agriculture,Guangxi University,Nanning,530004;School of Land Resources and Environment,Jiangxi Agricultural University/Innovative Integration Center of Industry and Education in Resource Utilization of Agricultural Waste,Prevention and Control of Non-point Pollution of Jiangxi Province,Nanchang,330045;Institute of Bioengineering,Guangdong Academy of Sciences/Guangdong Modern Agricultural Technology Research and Development Center(Resources and Environment and Agricultural Product Safety),Guangzhou,510316)

机构地区：[1]广西大学农学院,亚热带农业生物资源保护与利用国家重点实验室,甘蔗生物学广西重点实验室,南宁530004 [2]江西农业大学国土资源与环境学院,江西省农业废弃物资源化利用与面源污染防控产教融合重点创新中心,南昌330045 [3]广东省科学院生物工程研究所,广东省现代农业产业技术研发中心(资源环境与农产品安全),广州510316

出　　处：《基因组学与应用生物学》2022年第1期128-139,共12页Genomics and Applied Biology

基　　金：国家自然科学基金项目(31660592);广西自然科学基金项目(2015GXNSFBA139071)共同资助。

摘　　要：磷是植物生长发育重要的大量营养元素之一。甘蔗主产区酸性红壤磷素营养缺乏,严重影响了甘蔗的生长及产量的提高。本研究利用磷吸收利用效率基因型差异显著的两个甘蔗品种ROC22和Phil72-445为材料,通过苗期营养液培养法进一步评价与验证了ROC22和Phil72-445甘蔗磷效率的基因型差异特征,以及通过同源克隆法克隆甘蔗磷转运子Pht1家族基因并采用荧光定量PCR技术分析其表达模式。结果显示:低磷胁迫下,ROC22具有比Phil72-445显著强势的生物量、植株磷累积量、地上部磷转移系数,表现为磷吸收转运高效基因型;采用同源克隆法得到甘蔗磷转运蛋白Pht1家族的6条成员基因序列(SoPht1;2,SoPht1;3,SoPht1;5,SoPht1;6,SoPht1;11,SoPht1;12),生物信息学分析得知其与高粱、稻、玉蜀黍等的Pht1家族基因序列高度相似;低磷胁迫后期(处理31 d),SoPht1;2,SoPht1;5和SoPht1;6在ROC22和Phil72-445中均有明显的低磷诱导增强表达,且这三个基因在下部成熟叶(正四叶)中的诱导增强表达很可能对ROC22的磷高效起到主要作用。本研究结果为进一步系统深入研究SoPht1基因在甘蔗磷高效中的功能机理提供了依据。Phosphorus(P)is one of the most important macronutrient elements in plant.P deficiency in the main producing areas of sugarcane has seriously influence on the growth and yield of sugarcane.In this study,the genotypic differences of P efficiency between ROC22 and Phil72-445 screened from our previous research were further evaluated and verified by hydroponic cultivation at seedling stage.Sugarcane P transporter Pht1 family genes were cloned by homology cloning,and their functional expression patterns in ROC22 and Phil72-445 were analyzed by RT-qPCR.The results were as follow:compared with Phil72-445,ROC22 showed significant advantages in the biomass,P accumulation and shoot P transfer ratio under low P stress,which indicated that ROC22 acted as a P efficient genotype in P uptake and transport;six gene sequences(SoPht1;2,SoPht1;3,SoPht1;5,SoPht1;6,SoPht1;11,SoPht1;12)of Pht1 family were obtained by homologous cloning,which were highly similar to the Pht1 family gene sequences of sorghum,rice,corn,etc.based on the bioinformatics analysis;the enhanced expression of SoPht1;2,SoPht1;5 and SoPht1;6 in both ROC22 and Phil72-445 were induced significantly by low P stress in the later stage(treated for 31 d),and more enhanced expression in ROC22 than Phil72-445,while the expression of the three genes in the lower mature leaves(the fully expanded 4 th leaves)possibly mainly contribute to P efficiency in P-efficient cultivar ROC22.These results provide a basis for further and systematic research of the functional mechanism of SoPht1 genes for P efficiency in sugarcane.

关 键 词：甘蔗 磷效率 磷转运子 同源克隆法 表达分析 

分 类 号：S566.1[农业科学—作物学]