胚胎培养液和囊胚腔液中基因组DNA在胚胎植入前遗传学检测中的研究进展  

Research progress of genomic DNA in spent embryo culture media and the blastocoel fluid in preimplantation genetic testing

作　　者：王晓丹 苗穗兵 吴小华[1] Wang Xiaodan;Miao Suibing;Wu Xiaohua(The Fourth Hospital of Shijiazhuang(Obstetrics and Gynecology Affiliated Hospital of Hebei Medical University),Shijiazhuang 050000,China)

机构地区：[1]石家庄市第四医院(河北医科大学附属妇产医院),石家庄050000

出　　处：《中华生殖与避孕杂志》2022年第3期311-317,共7页Chinese Journal of Reproduction and Contraception

摘　　要：胚胎染色体异常是导致妊娠失败的主要原因之一,行胚胎植入前遗传学检测(preimplantation genetic testing,PGT),选择整倍体胚胎移植,可显著提高胚胎种植率和持续妊娠率。但由于PGT技术对胚胎的侵入性和技术的复杂性,限制了其在临床的广泛应用,开发一种安全、快速、经济的胚胎基因组检测方法将是生殖领域的一大进步。近年来,随着游离基因组DNA在胚胎培养液和囊胚腔液中的发现,许多学者开始探讨胚胎游离DNA在PGT中的适用性,然而目前的研究结果缺乏一致性结论。本文总结了胚胎培养液和囊胚腔液中基因组DNA在PGT领域的研究进展,并讨论其当前的局限性以及应用前景。Chromosomal abnormality of embryos is one of the main causes of pregnancy failure.Preimplantation genetic testing(PGT)can significantly improve the implantation rate and the rate of continuous pregnancy by selecting chromosomally normal embryos to transfer,but the invasive nature and operational complexity of PGT limit its clinical application.Therefore,the development of a non-invasive,rapid and economical method for embryo genome detection would represent a progress in assisted reproduction.In recent years,with the discovery of cell-free genomic DNA in spent embryo culture media and the blastocoel fluid,genetic analysis of cell-free DNA has been reported in evaluating the genetic constitution of embryos,however,the published studies lack consistent conclusions.In this review,we have summarized the progress of cell-free genomic DNA in PGT and discussed its current limitations and future clinical implications.

关 键 词：游离核酸 胚胎植入前遗传学检测 胚胎培养液 囊胚腔液 

分 类 号：R714.8[医药卫生—妇产科学]