探讨PLK1在卵母细胞减数分裂中的调控机制及其与有丝分裂的异同  

Research on the regulatory mechanism of PLK1 in oocyte meiosis and its similarities and differences between meiosis and mitosis

作　　者：姚波 刘凤 莫晓龙 任艳萍 Yao Bo;Liu Feng;Mo Xiaolong;Ren Yanping(Department of Histology and Embryology,School of Basic Medicine,Zunyi Medical University,Zunyi 563000,China)

机构地区：[1]遵义医科大学基础医学院组织胚胎学教研室,遵义563000

出　　处：《中华生殖与避孕杂志》2022年第3期318-323,共6页Chinese Journal of Reproduction and Contraception

基　　金：国家自然科学基金项目(31860329);贵州省科技厅项目(黔科合基础[2019]1344号);贵州省教育厅科技青年人才成长项目(黔教合KY字[2017]183)。

摘　　要：Polo样激酶1(Polo-like kinase 1,PLK1)是丝氨酸/苏氨酸蛋白激酶家族成员之一。在有丝分裂中,PLK1参与控制中心体成熟、染色体凝聚、动粒-微管附着和胞质分裂。在哺乳动物卵母细胞减数分裂中PLK1也具有与在有丝分裂中类似的功能,但尚缺少PLK1在卵母细胞减数分裂调节机制方面的系统性的文献综述报道。本文从PLK1在卵母细胞减数分裂各时期的定位情况,及其在卵母细胞染色体凝聚、微管组织中心解聚、γ-微管蛋白和中心粒周蛋白的募集以及后期促进复合物/细胞周期体(anaphase-promoting complex/cyclostome,APC/C)的活化中的作用,这几个方面较为系统地探讨PLK1在卵母细胞减数分裂中的分子机制,及其在有丝分裂与减数分裂之间的异同,为进一步解析卵母细胞减数分裂的分子机制提供理论基础。Polo-like kinase 1(PLK1)is one of the members of the serine/threonine protein kinase family.In mitosis,PLK1 is involved in regulating centrosome maturation,chromosome condensation,kinetochore-microtubule attachment,and cytokinesis.It has been pointed out in some researches that PLK1 also has similar functions in meiosis in mammalian oocytes,but there is no systematic review on the role of PLK1 in the mechanism of meiosis regulation in oocytes.In this study,the regulating mechanism of PLK1 in oocyte meiosis and the similarities and differences between meiosis and mitosis were investigated systematically by the location of PLK1 in oocyte,and the role of PLK1 in chromosome condensation,microtubule organizing centers(MTOCs)depolymerization,the recruitment ofγ-tubulin and Pericentrin,and activation of anaphase-promoting complex/cyclostome(APC/C).It will lay the foundations for the further research on the molecular mechanism of meiosis in oocytes.

关 键 词：PLK1 卵母细胞 减数分裂 微管组织中心 纺锤体形成 

分 类 号：R339.22[医药卫生—人体生理学]