代谢综合征大鼠主动脉Apelin及其受体的表达及意义  

The Expression and Significance of Apelin and Its Receptor on the Aorta in Metabolic Syndrome Model Rats

作　　者：樊小容 张旭升[1] 黄战军[1] 谭小青 蔡博治 FAN Xiaorong;ZHANG Xusheng;HUANG Zhanjun;TAN Xiaoqing;CAI Bozhi(Department of Cardiology,Longgang People’s Hospital of Shenzhen,Guangdong Province,Shenzhen 518172,China;Laboratory of Molecular Cardiology,the First Affiliated Hospital of Medical School,Shantou University,Guangdong Province,Shantou 515000,China)

机构地区：[1]深圳市龙岗区人民医院心内科,广东深圳518172 [2]汕头大学医学院第一附属医院分子心脏病学实验室,广东汕头515000

出　　处：《中国中医药现代远程教育》2022年第14期148-150,共3页Chinese Medicine Modern Distance Education of China

摘　　要：目的观察代谢综合征(MS)大鼠主动脉Apelin及其受体(APJ)的表达变化。方法16只雄性SD大鼠按随机数字表法均分为对照组和MS组,每组8只。MS组采用高果糖饲料(60%)喂养,对照组采用常规饲料喂养,喂养周期为10周。采用Masson染色法检测血管胶原沉积情况,酶联免疫吸附法检测大鼠血清和主动脉组织Apelin含量,免疫组化法检测大鼠主动脉组织APJ的表达。结果高果糖饮食可成功诱导高血压、高三酰甘油(TG)血症和高血糖等特征的代谢综合征模型,MS组大鼠主动脉胶原沉积明显,血清Apelin浓度和主动脉组织Apelin/APJ表达较对照组明显下调(P<0.05)。结论MS大鼠主动脉发生重构,Apelin/APJ表达下调。提示Apelin可能为新的血管保护因子。Objective To investigate the expression of Apelin and its receptor(APJ)on the aorta of rats with metabolic syndrome(MS).Methods Sixteen SD rats were randomly divided into control group and experimental group,with 8 rats in each group.The experimental group(MS group)was fed with high fructose diet(60%),and the control group was fed with conventional diet.The feeding cycle was 10 weeks.The expression of collagen was determined by Masson staining,Apelin contents in serum and aorta were determined by elisa,and the expressions of APJ were determined by immunohistochemistry.Results The MS model of rats were induced by high fructose diet successfully,with hypertension,hypertriglyceridemia and hyperglycemia.Masson staining showed that there were collagen deposited in aortic wall of MS rats.The contents of Apelin in serum and the expressions of Apelin/APJ in aorta were obviously down-regulated in MS group(P<0.05).Conclusion Vascular remodeling occurred in MS rats,and the Apelin and APJ were significantly down-regulated.It is suggested that Apelin may be a new vascular protective factor.

关 键 词：代谢综合征 APELIN APJ受体 血管重构 大鼠 

分 类 号：R589[医药卫生—内分泌]