高迁移率族蛋白B1 PCT TGF-β1在新生儿肺炎中的表达水平及意义  

Expression Level and Significance of High Mobility Group Proteins B1 PCT and TGF-β1 in Neonatal Pneumonia

作　　者：王芳[1] 彭湘莲[1] 王玲华[1] 杨玉香[1] 郭映林 刘银燕 WANG Fang;PENG Xianglian;WANG Linghua(Hunan Maternal and Child Health Centre,Hunan Changsha 410008,China)

机构地区：[1]湖南省妇幼保健院新生儿一科,湖南长沙410008

出　　处：《河北医学》2022年第7期1103-1107,共5页Hebei Medicine

基　　金：湖北省重点实验室开放基金课题编号,(编号:PTS2021-02)。

摘　　要：目的:分析高迁移率族蛋白B1(HMGB1)、降钙素原(PCT)、血管转化生长因子β1(TGF-β1)在新生儿肺炎中的表达水平及意义。方法:选取2020年1月至2021年1月我院收治的新生儿肺炎患儿80例作为新生儿肺炎组,根据是否为细菌感染性肺炎分为非细菌性感染组、细菌性感染组,根据疾病严重程度分为轻症组34例、中症型25例、重症组21例,根据疾病恢复情况分为急性期组50例、缓解期组30例。另外选择同期于我院新生儿科健康新生儿80例,作为健康对照组。抽取所有研究对象静脉血,并采用双抗体夹心ELISA法测定HMGB1、TGF-β1表达水平,以及采用飞测免疫荧光检测仪检测PCT表达水平,并分析其相关性。结果:新生儿肺炎组血清HMGB1、PCT、TGF-β1表达水平均高于健康对照组(P<0.05)。细菌性感染组患儿血清HMGB1、PCT、TGF-β1表达水平均高于非细菌性感染组(P<0.05)。重症组患儿血清HMGB1、PCT、TGF-β1表达水平均高于轻症组和中症组(P<0.05)。急性期组患儿血清HMGB1、PCT、TGF-β1表达水平均高于恢复期组(P<0.05)。HMGB1与PCT正相关(r=0.261,P=0.019);HMGB1与TGF-β1正相关(r=0.245,P=0.027);PCT与TGF-β1正相关(r=0.479,P=0.001)。结论:HMGB1、PCT、TGF-β1在新生儿肺炎中高表达,与患儿细菌性感染、严重程度、疾病转归相关,且三者呈现为正相关,共同参与此病的发生发展。Objective:To analyze the expression levels and significance of HMGB1,PCT and TGF-β1 in neonates with pneumonia.Methods:Eighty children with neonatal pneumonia admitted to our hospital from January 2020 to January 2021 were selected as the neonatal pneumonia group.According to the bacterial infection or not,they were divided into non-bacterial infection group and bacterial infection group,and according to the severity of the disease,they were divided into mild group(34 cases),moderate syndrome group(25 cases)and severe group(21 cases).According to the disease recovery,the patients were divided into acute stage group(50 cases)and remission stage group(30 cases).In addition,80 healthy neonates in the Department of Neonatology of our hospital were selected as healthy control group.The venous blood of all subjects was extracted,and the expression levels of HMGB1 and TGF-β1 were determined by double-antibody sandwich ELISA method,and the expression levels of PCT were detected by flying immunofluorescence detector,and their correlation was analyzed.Results:The expression levels of SERUM HMGB1,PCT and TGF-β1 in neonatal pneumonia group were higher than those in healthy control group(P<0.05).The expression levels of SERUM HMGB1,PCT and TGF-β1 in the bacterial infection group were higher than those in the non-bacterial infection group(P<0.05).The levels of serum HMGB1,PCT and TGF-β1 in severe group were higher than those in mild and moderate group(P<0.05).The expression levels of SERUM HMGB1,PCT and TGF-β1 in acute group were higher than those in recovery group(P<0.05).HMGB1 was positively correlated with PCT(r=0.261,P=0.019).HMGB1 was positively correlated with TGF-β1(r=0.245,P=0.027).PCT was positively correlated with TGF-β1(r=0.479,P=0.001).Conclusion:The high expression of HMGB1,PCT and TGF-β1 in neonatal pneumonia was positively correlated with bacterial infection,severity and disease outcome,and all three were involved in the occurrence and development of this disease.

关 键 词：高迁移率族蛋白B1 降钙素原 血管转化生长因子β1 新生儿肺炎 

分 类 号：R722.135[医药卫生—儿科]