悬钩子根多糖对2型糖尿病小鼠胰腺线粒体功能的改善作用  

Improvement effect of Rubus root polysaccharide on pancreatic mitochondrial function in type 2 diabetic mice

作　　者：代敏敏 常影 许娜 王燕[3] 徐梦 徐文悦 马佳旺 刘文森[3] 陈正爱[1] DAI Minmin;CHANG Ying;XU Na;WANG Yan;XU Meng;XU Wenyue;MA Jiawang;LIU Wensen;CHEN Zhengai(Department of Pharmacology,School of Basic Medical Sciences,Yanbian University,Yanji 133002,China;Department of Pharmacology,School of Pharmacy,Jilin Medical University,Jilin 132013,China;Institute of Military Veterinary Medicine,Academy of Military Medical Sciences,Changchun 130118,China;Department of Biochemistry and Molecular Biology,School of Life Sciences,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]延边大学基础医学院药理学教研室,吉林延吉133002 [2]吉林医药学院药学院药理学教研室,吉林吉林132013 [3]军事医学科学院军事兽医研究所,吉林长春130118 [4]吉林农业大学生命科学学院生物化学与分子生物学教研室,吉林长春130118

出　　处：《吉林大学学报（医学版）》2022年第4期938-945,共8页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅重点研发项目(20180201033YY)。

摘　　要：目的:探讨悬钩子根多糖(RRP)对2型糖尿病(T2DM)小鼠胰腺线粒体功能的改善作用,并阐明其作用机制。方法:70只健康雄性C57BL/6J小鼠适应性饲养1周,随机选取10只小鼠作为对照组,其余60只小鼠按照高脂肪饮食联合腹腔注射低剂量链脲佐菌素(STZ)的方法建立T2DM小鼠模型。从52只造模成功的小鼠中随机选取50只小鼠分为模型组,低、中和高剂量RRP组,二甲双胍组,每组10只。每日1次连续10周灌胃给药,对照组和模型组小鼠灌胃等体积生理盐水。实验过程中每日观察小鼠的一般状态,每周监测空腹血糖(FBG)水平,每4周进行口服葡萄糖耐量试验(OGTT)。连续灌胃10周后,计算各组小鼠OGTT时间-血糖曲线下面积(AUC)和稳态模型胰岛素抵抗指数(HOMA-IR),检测各组小鼠血清中空腹胰岛素(FINS)、C肽、胰高血糖素(GC)水平和线粒体功能相关指标。取各组小鼠胰腺组织,计算胰腺指数,HE染色观察各组小鼠胰腺组织病理形态表现。结果:与对照组比较,模型组小鼠精神萎靡,毛发粗糙,自主活动减少;与模型组比较,各剂量RRP组和二甲双胍组小鼠精神状态较好,毛发有光泽,自主活动增加。与对照组比较,模型组小鼠体质量明显降低(P<0.05),饮水量和摄食量明显增加(P<0.05),血清FBG水平、OGTT的AUC和HOMA-IR明显升高(P<0.05);与模型组比较,中和高剂量RRP组及二甲双胍组小鼠体质量明显增加(P<0.05),饮水量和摄食量明显减少(P<0.05),OGTT的AUC明显降低(P<0.05),各剂量RRP组小鼠血清FBG水平和HOMA-IR均明显降低(P<0.05)。与对照组比较,模型组小鼠血清FINS和C肽水平明显降低(P<0.05),GC水平明显升高(P<0.05),磷氧比(ADP/O)、呼吸控制率(RCR)、基础呼吸耗氧量和胰腺指数均明显降低(P<0.05);与模型组比较,中和高剂量RRP组及二甲双胍组小鼠血清FINS和C肽水平明显升高(P<0.05),GC水平明显降低(P<0.05),ADP/O和RCR明显升高(P<0.05),各剂量RRP组和二甲Objective:To investigate the improvement effect of Rubus root polysaccharide(RRP)on the pancreatic mitochondrial function in the type 2 diabetes mellitus(T2DM)mice,and to clarify its mechanism.Methods:A total of 70 healthy male C57BL/6J mice were adaptively fed for 1 week,10 mice were randomly selected as control group,and the remaining 60 mice were used to establish T2DM mouse model according to the method of high-fat diet combined with intraperitoneal injection of low dose streptozotocin(STZ).Fifty mice were randomly selected from 52 mice that were successfully modeled and divided into model group,low,medium,and high doses of RRP groups,and metformin group;with 10 mice in each group.The mice were administered once a day for 10 weeks;the mice in control group and model group were given equal volumes of normal saline by gavage.During the experiment,the general state of mice was observed every day,the fasting blood glucose(FBG)level was monitored weekly,and oral glucose tolerance test(OGTT)was performed every 4 weeks.After continuous treatment for 10 weeks,the area under time-glucose curve(AUC)of OGTT and the insulin resistance index(HOMA-IR)of the mice in various groups were calculated.The levels of serum fasting insulin(FINS),C-peptide,and glucagon(GC)and the pancreatic mitochondrial function-related indicators were detected.The pancreas tissue of mice in various groups was obtained,the pancreatic indexes were calculated,and the pathomorphology of pancreas tissue of mice in various was observed by HE staining.Results:Compared with control group,the mice in model group were apathetic,with rough hair and decreased voluntary activity;compared with model group,the mice in different doses of RRP groups and metformin group had better mental state,with shiny hair and increased voluntary activities.Compared with control group,the body mass of the mice in model group was significantly decreased(P<0.05),the amount of drinking water and food intake were significantly increased(P<0.05),and the serum FBG level,AUC of OGTT an

关 键 词：悬钩子根多糖 糖尿病 2型 胰腺 线粒体功能 

分 类 号：R285.5[医药卫生—中药学] R587.1[医药卫生—中医学]