三(2-氯乙基)磷酸酯(TCEP)对大菱鲆(Scophthalmus maximus)免疫相关基因表达的影响  

Effects of TCEP on Immune-related Functional Gene in Scophthalmus maximus

作　　者：刘聪 衣丹 柴迎梅[1] 丛柏林 LIU Cong;YI Dan;CHAI Ying-mei;CONG Bai-lin(Marine College,Shandong University,Weihai 264200,China;First Institute of Oceanography,MNR,Qingdao 266061,China)

机构地区：[1]山东大学海洋学院,山东威海264200 [2]自然资源部第一海洋研究所,山东青岛266061

出　　处：《海洋科学进展》2022年第3期473-483,共11页Advances in Marine Science

基　　金：中央级公益性科研院所基本科研业务费专项资金资助项目——内分泌干扰物对海水养殖大菱鲆的生物学效应研究(GY0218Q03)。

摘　　要：三(2-氯乙基)磷酸酯(Tris(2-carboxyethyl)phosphine,TCEP)是一种新型内分泌干扰物(Endocrine Disrupting Chemicals,EDCs),低剂量就会造成很强的生物毒性。通过研究不同质量浓度梯度的TCEP对养殖大菱鲆的胁迫,发现其在24 h、48 h、72 h和96 h的半致死浓度(LC_(50))分别为190.76 mg/L、159.94 mg/L、140.70 mg/L和110.71 mg/L;安全浓度(SC)为33.60 mg/L。24 h内不同质量浓度梯度的TCEP和不同胁迫时间对养殖大菱鲆的影响表明,高于质量浓度33.60 mg/L的TCEP对大菱鲆具有急性毒性效应。用实时荧光定量PCR技术分析TCEP急性胁迫的养殖大菱鲆体内2种细胞免疫因子−组织相容性复合体alpha(MHC-Ⅱα)和肿瘤坏死因子alpha(TNF-α)的基因相对表达水平,发现经TCEP胁迫后2种细胞免疫因子的表达量都显著上调;随TCEP的质量浓度升高,特别是达到47.69 mg/L(LC_(50))以上时表达量的变化更加明显;而胁迫时间超过12 h,基因表达量会呈现先上升后下降的趋势,说明对大菱鲆免疫系统造成急性损伤。研究结果可为大菱鲆健康养殖提供参考,并为环境污染物检测提供分子标记参考。Tris(2-carboxyethyl)phosphine(TCEP)is a new endocrine disruptor(Endocrine Disrupting Chemicals,EDCs),which can cause strong biological toxicity at low doses.With the increasing use and sewage of TCEP entering the sea through rivers,its accumulation in seawater and its threat to aquatic organisms are increasing.Turbot(Scophthalmus maximus)is a common large-scale mariculture fish,the TCEP accumulated in seawater will affect the development of its aquaculture industry.By studying the effects of different concentrations gradient and different stress times of TCEP on turbot,LC_(50) at four time points were 190.76 mg/L(24 h),159.94 mg/L(48 h),140.70 mg/L(72 h)and 110.71 mg/L(96 h),and safe concentration(SC)was 33.60 mg/L.This provided a basis for healthy culture and toxicologic research of the burbot.Using real-time fluorescence quantitative PCR technique,the relative expression levels of two cytokines,histocompatibility complexα(MHC-IIα)and tumor necrosis factorα(TNF-α),were analyzed.It was found that the expressions of these cytokines were up-regulated after TCEP treatment;With the increase of mass concentration of TCEP,especially it reached 47.69 mg/L(LC_(50)),the changes of gene expressions were more obvious;While the stress time was more than 12 h,the gene expressions showed a trend of increasing first and decreasing afterward.It showed that TCEP can cause acute injury to the immune system of turbot.This study can provide the reference for both the healthy culture of turbot and a molecular marker for detection of environmental pollutants.

关 键 词：三(2-氯乙基)磷酸酯(TCEP) 大菱鲆 半致死浓度(LC_(50)) 免疫相关基因 

分 类 号：P735[天文地球—海洋生物学] Q754[天文地球—海洋科学]