基于Ag-CDs纳米粒子的比色法和SERS方法协同检测尿酸  

Determination of Uric Acid Using Colorimetric Method and SERS Method Based on Ag-CDs Nanoparticles

作　　者：何婧 于海波[1] 王艾琳 HE Jing;YU Haibo;WANG Ailin(School of Physics,Northeast Normal University,Changchun 130024,China)

机构地区：[1]东北师范大学物理学院,长春130024

出　　处：《吉林大学学报（理学版）》2022年第4期962-969,共8页Journal of Jilin University:Science Edition

基　　金：吉林省科技厅国际合作项目(批准号:20210402062GH).

摘　　要：用碳量子点(CDs)还原Ag离子,得到核壳结构的Ag-CDs纳米粒子.结果表明:Ag核的直径约为30 nm,表面碳膜厚度约为2.2 nm;Ag-CDs纳米粒子具有优异的类过氧化物酶活性以及表面增强Raman散射(SERS)活性,可催化3,3′,5,5′-四甲基联苯胺(TMB)氧化,由于尿酸可将氧化的TMB还原,因此可通过比色法检测尿酸的浓度;以Ag-CDs纳米粒子为SERS基底,通过SERS光谱可检测尿酸浓度,两种方法协同检测可大幅度提高检测的可信度.Ag-CDs nanoparticles with core-shell structure were obtained by reducing Ag ion with carbon dots(CDs).The results show that the diameter of Ag core is about 30 nm,and the thickness of surface carbon film is about 2.2 nm.Ag-CDs nanoparticles have excellent peroxidase-like activity and surface enhanced Raman scattering(SERS)activity,which can catalyze the oxidation of 3,3′,5,5′-teramethylbenzidine(TMB).Because uric acid can reduce the oxidized TMB,the concentration of uric acid can be detected by colorimetry.Taking Ag-CDs nanoparticles as the SERS substrate,and the concentration of uric acid can be detected by SERS spectrum.The collaborative detection of the two methods can greatly improve the reliability of the detection.

关 键 词：类过氧化物酶 核壳结构纳米粒子 表面增强Raman 尿酸 

分 类 号：O469[理学—凝聚态物理]