柑橘黑点病菌种群对嘧菌酯敏感性基线的建立  

Establishment of the sensitivity baseline of Diaporthe citri population to azoxystrobin

作　　者：曾雅婷 熊桃 柏自琴[2] 谭振华 李红叶[1] ZENG Yating;XIONG Tao;BAI Ziqin;TAN Zhenhua;LI Hongye(Institute of Biotechnology,Zhejiang University,Hangzhou 310058,China;Institute of Fruit Science,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China;Agricultural and Rural Affairs Bureau of Yizhang County,Hunan Province,Chenzhou 424200,China)

机构地区：[1]浙江大学生物技术研究所,杭州310058 [2]贵州省农业科学院果树科学研究所,贵阳550006 [3]湖南省宜章县农业农村局,郴州424200

出　　处：《植物保护》2022年第4期138-142,共5页Plant Protection

基　　金：浙江省重点研发计划(2019C02022);国家现代农业(柑橘)产业技术体系建设专项(CARS-26)。

摘　　要：为明确柑橘黑点病菌Diaporthe citri对嘧菌酯的敏感性以及嘧菌酯在我国柑橘黑点病防治中的应用潜力,分别采用菌丝生长抑制法和孢子萌发抑制法测定了来自浙江、湖南、江西、贵州4个主要柑橘产区的66个柑橘黑点病菌菌株对嘧菌酯的敏感性,并建立了其种群对嘧菌酯的敏感性基线。结果显示:嘧菌酯对柑橘黑点病菌菌丝生长和孢子萌发的EC_(50)平均值分别为(0.2285±0.1138)μg/mL和(0.0256±0.0069)μg/mL,种群对嘧菌酯的敏感性频次分布曲线为连续的单峰型,符合正态分布。因此可将嘧菌酯对这66个菌株的菌丝生长和孢子萌发的平均EC_(50)作为柑橘黑点病菌对嘧菌酯的敏感性基线,该敏感性基线可作为监测田间柑橘黑点病菌对嘧菌酯敏感性变化的参考,对指导今后科学用药具有现实意义。同时,本研究所使用的66个菌株对嘧菌酯敏感性均较高,没有抗性菌株出现,表明未来可以将嘧菌酯用于柑橘黑点病的防治,作为黑点病防治主打药剂代森锰锌的一种补充。In order to clarify the sensitivity of Diaporthe citri,the pathogen of citrus melanose,to azoxystrobin and the application potential of azoxystrobin in the control of citrus melanose in China,the sensitivity of 66 strains of D.citri from four main citrus producing areas,Zhejiang,Hunan,Jiangxi and Guizhou provinces,to azoxystrobin was determined by mycelial growth inhibition and spore germination inhibition,respectively,and the sensitivity baseline of D.citri population to azoxystrobin was established.The results showed that the average EC_(50) values of azoxystrobin to D.citri mycelial growth and spore germination were(0.2285±0.1138)μg/mL and(0.0256±0.0069)μg/mL,respectively.The frequency distribution curve of sensitivity of all strains of D.citri to azoxystrobin was continuous unimodal and close to normal distribution.Therefore,the average EC_(50) values of mycelial growth and spore germination can be used as the sensitivity baseline of D.citri to azoxystrobin.This sensitivity baseline could be used to monitor the changes of sensitivity of D.citri to azoxystrobin in the citrus orchards,and has a practical significance for guiding the use of azoxystrobin in melanose control in the future.The 66 strains of D.citri tested in this study were all highly sensitive to azoxystrobin,and no resistant strains were found,indicating that azoxystrobin could be used in the prevention and control of citrus melanose in the future,as a supplement of mancozeb,the main fungicide used in citrus melanose management.

关 键 词：柑橘黑点病菌 嘧菌酯 敏感性基线 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]