番茄褪绿病毒病在山东设施西葫芦上的发生及潜在危害  

Occurrence of tomato chlorosis virus and its potential threat to greenhouse zucchinis in Shandong province

作　　者：代惠洁[1] 燕颖 赵静 乔宁[1] 张德珍[1] 竺晓平 孙小桉 DAI Huijie;YAN Ying;ZHAO Jing;QIAO Ning;ZHANG Dezhen;ZHU Xiaoping;SUN Xiaoan(Weifang University of Science&Technology,Shandong Facility Horticulture Bioengineering Research Center Horticulture,Shouguang 262700,China;Key Laboratory of Biology and Molecular Biology in University of Shandong,College of Biological and Agricultural Engineering,Weifang University,Weifang 261061,China;Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests,College of Plant Protection,Shandong Agricultural University,Tai’an 271018,China)

机构地区：[1]潍坊科技学院,山东省设施园艺生物工程研究中心,寿光262700 [2]潍坊学院生物与农业工程学院,山东省生物化学与分子生物学高校重点实验室,潍坊261061 [3]山东农业大学植物保护学院,山东省蔬菜病虫生物学重点实验室,泰安271018

出　　处：《植物保护》2022年第4期258-263,共6页Plant Protection

基　　金：山东省重点研发计划(2019GSF109118,2019GX074);潍坊科技学院学科建设专项(2021XKJS30);潍坊科技学院博士基金(2021KJBS08)。

摘　　要：番茄褪绿病毒tomato chlorosis virus(ToCV)是我国蔬菜生产的重要新发病毒,其寄主范围逐年扩大。2019年10月项目组在山东寿光西葫芦温室调查时发现部分叶片呈现黄化、脉间褪绿,类似于ToCV侵染症状,并伴有烟粉虱发生。利用特异性引物检测发现,扩增的目的片段与GenBank中登录的侵染番茄的ToCV基因序列(登录号:KC887998.1)同源性高达99.58%,充分说明西葫芦植株已被ToCV侵染。通过对病毒病发生规律和烟粉虱虫口数量调查发现,西葫芦定植后1个月表现侵染症状的植株为2.0%,定植后2个月达到4.2%,定植后4个月后高达68.2%,病毒发生呈指数增长,而烟粉虱虫口数量却维持较低密度。从济南和德州采集的西葫芦疑似病叶和烟粉虱中也检出ToCV病毒,说明该病毒可能已经在山东省设施西葫芦主要种植区普遍发生,并经烟粉虱广泛传播,需引起高度重视。Tomato chlorosis virus(ToCV)has become a new important virus in vegetable industry in China and its host range keeps expanding.In October,2019,yellow leaves and chlorosis between veins similar to the symptoms of tomato caused by ToCV infections were noticed on zucchini plants in a greenhouse located in Shouguang county.The gene fragment amplified using a pair of specific primers was cloned,sequenced and BLAST in GenBank.The results showed that its sequence shared a high similarity(99.58%)with that of ToCV registered in GenBank(Acc.no.KC887998.1),indicating that zucchini plants were commonly infected by ToCV.A preliminary investigation in the greenhouse in Shouguang indicated that the percentage of symptomatic zucchinis was 2.0%in a month,4.2%in two months,and 68.2%in four months,respectively,after planting,and an exponential development of ToCV was observed,while the number of Bemisia tabaci remained at a low level.ToCV was also detected in the suspected leaves of zucchini and B.tabaci collected from Jinan and Dezhou cities.The results suggested that ToCV had already spread widely in the areas where zucchinis were cultivated in greenhouse in Shandong province,which should to be taken seriously.

关 键 词：西葫芦 番茄褪绿病毒 烟粉虱 虫口数量 

分 类 号：S436.429[农业科学—农业昆虫与害虫防治]