AgInS_(2)/ZnS quantum dots for noninvasive cervical cancer screening with intracellular pH sensing using fluorescence lifetime imaging microscopy  

作　　者：Wenhua Su Dan Yang Yulan Wang Yawei Kong Wanlu Zhang Jing Wang Yiyan Fei Ruiqian Guo Jiong Ma Lan Mi 

机构地区：[1]Department of Optical Science and Engineering,Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing,Key Laboratory of Micro and Nano Photonic Structures(Ministry of Education),School of Information Science and Technology,Fudan University,Shanghai 200433,China [2]Institute for Electric Light Sources,School of Information Science and Technology,Fudan University,Shanghai 200433,China [3]Department of Gynecology and Obstetrics,The Central Hospital of Wuhan,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430014,China [4]Institute of Photonic Chips,University of Shanghai for Science and Technology,Shanghai 200093,China Centre for Artificial-Intelligence Nanophotonics,School of Optical-Electrical and Computer Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China [5]Institute of Biomedical Engineering and Technology,Academy for Engineer and Technology,Fudan University,220 Handan Road,Shanghai 200433,China [6]Shanghai Engineering Research Center of Industrial Microorganisms,the Multiscale Research Institute of Complex Systems(MRICS),School of Life Sciences,Fudan University,220 Handan Road,Shanghai 200433,China

出　　处：《Nano Research》2022年第6期5193-5204,共12页纳米研究（英文版）

基　　金：supported by the National Natural Science Foundation of China(NSFC,Nos.62074044,61904036,and 11804350);the Medical Engineering Fund of Fudan University(No.yg2021-022);Zhongshan-Fudan Joint Innovation Center and Jihua Laboratory Projects of Guangdong Province(No.X190111UZ190);Fudan University-CIOMP Joint Fund(No.FC2018-001);Pioneering Project of Academy for Engineering and Technology of Fudan University(Nos.gyy2018-001 and gyy2018-002);Shanghai Natural Science Foundation(Nos.20ZR1405100 and 20ZR1403700);Science and Technology Research Program of Shanghai(No.19DZ2282100);Shanghai key discipline construction plan(2020-2022)(No.GWV-10.1-XK01);Shanghai Hong Kong,Macao,and Taiwan Cooperation Project(No.19490760900);Shanghai Engineering Technology Research Center of Hair Medicine(No.19DZ2250500).

摘　　要：Intracellular pH plays a critical role in biological functions,and abnormal pH values are related to various diseases.Here,we report on an intracellular pH sensor AgInS_(2)(AIS)/ZnS quantum dots(QDs)that show long fluorescence lifetimes of hundreds of nanoseconds and low toxicity.Fluorescence lifetime imaging microscopy(FLIM)combined with AIS/ZnS QDs is used for the imaging of live cells in different pH buffers and different cell lines.The FLIM images of AIS/ZnS QDs in live cells demonstrate different intracellular pH values in different regions,such as in lysosomes or cytoplasm.This method can also distinguish cancer cells from normal cells,and the fluorescence lifetime difference of the AIS/ZnS QDs between the two types of cells is 100±7 ns.Most importantly,the exfoliated cervical cells from 20 patients are investigated using FLIM combined with AIS/ZnS QDs.The lifetime difference value between the normal and cervical cancer(CC)groups is 115±9 ns,and the difference between the normal and the precancerous lesion group is 64±9 ns.For the first time,the noninvasive method has been used for cervical cancer screening,and it has shown great improvement in sensitivity compared with a clinical conventional cytology examination.

关 键 词：AIS/ZnS quantum dots fluorescence lifetime imaging microscopy intracellular pH sensing cervical cancer screening NONINVASIVE 

分 类 号：TB3[一般工业技术—材料科学与工程]