肺瘤平膏调控肿瘤炎性微环境NF-κB信号通路抑制Lewis肺癌的效应机制  

Effect mechanism of Feiliuping Ointment on inhibition of Lewis lung cancer by regulating NF-κB signaling pathway in tumor inflammatory microenvironment

作　　者：陈雪 何善泉 徐心瑶 张葛[3] 刘瑞[3] 花宝金[1] 李卫东[2] CHEN Xue;HE Shan-quan;XU Xin-yao;ZHANG Ge;LIU Rui;HUA Bao-jin;LI Wei-dong(Guang’anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China;Department of Scientific Research Management,Guang’anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053;Department of Oncology,Guang’anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053)

机构地区：[1]中国中医科学院广安门医院,北京100053 [2]中国中医科学院广安门医院科研处,北京100053 [3]中国中医科学院广安门医院肿瘤科,北京100053

出　　处：《北京中医药》2022年第5期484-489,共6页Beijing Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81102587)。

摘　　要：目的基于肿瘤炎性微环境NF-κB信号通路及炎性细胞因子,探究肺瘤平膏防治肺癌的效应机制。方法采用SPF级C57BL/6雄性小鼠进行Lewis肺癌移植瘤造模,分为空白对照组、模型对照组、肺瘤平膏组、环磷酰胺(Cyclophos-phamide,CTX)组、肺瘤平膏+CTX组共5组。肺瘤平膏组给予肺瘤平膏灌胃,CTX组给予CTX腹腔注射,肺瘤平膏+CTX组给予以上2种干预措施。各组均干预21 d后处死,称瘤体质量,计算抑瘤率;免疫组织化学法检测肿瘤组织中NF-κB、Ecadherin、N-cadherin表达情况;ELISA法检测小鼠血清中炎性细胞因子IL-6、TNF-α和IL-1β的水平。结果肺瘤平膏可抑制Lewis肺癌移植瘤肿瘤生长达38.8%,而与CTX联合用药可抑制肿瘤生长达83.2%;与模型对照组比较,肺瘤平膏显著降低血清中促炎细胞因子IL-6、TNF-α和IL-1β的水平(P<0.01),抑制肿瘤组织中NF-κB、N-cadherin的表达,促进Ecadherin的表达(P<0.01)。结论肺瘤平膏可抑制肿瘤炎性微环境中关键信号NF-κB及炎性细胞因子水平,进而调控上皮间质转化,提示肺瘤平膏可能是炎性相关肺癌防治的有效方药。Objective To explore the effect mechanism of Feiliuping Ointment on the prevention and treatment of lung cancer based on NF-κB signaling pathways and inflammatory cytokines in tumor inflammatory microenvironment.Methods SPF grade C57 BL/6 male mice were modeled by Lewis lung cancer cell transplanted tumor,and divided into 5 groups,that is blank control group,model control group,Feiliuping Ointment group,cyclophosphamide(CTX)group,and CTX+Feiliuping Ointment group.The mice were given Feiliuping Ointment gavage or intraperitoneal injection of CTX for 21 days respectively,and then sacrificed.The tumor was weighed to calculate the tumor inhibition rate;the expression of NF-κB,E-cadherin and N-cadherin in tumor tissue was detected by immunohistochemistry;the levels of inflammatory cytokines IL-6,TNF-αand IL-1βin the serum of mice were detected by ELISA.Results Feiliuping Ointment can inhibit the growth of Lewis lung cancer xenograft tumor by 38.8%,while the combination with CTX can inhibit the tumor growth by 83.2%;compared with the model group,Feiliuping Ointment significantly reduced the proinflammatory cytokines IL-6,TNF-αand IL-1βin serum(P<0.01);compared with the model group,Feiliuping Ointment inhibited the expression of NF-κB,promoted the expression of E-cadherin and inhibited the expression of N-cadherin in tumor tissue(P<0.01).Conclusion Feiliuping Ointment can inhibit the NF-κB signaling pathway and the levels of inflammatory cytokines in tumor inflammatory microenvironment,thereby regulating epithelial-mesenchymal transition,suggesting that Feiliuping Ointment may be an effective prescription for the prevention and treatment of inflammation-related lung cancer.

关 键 词：肺瘤平膏 肿瘤炎性微环境 NF-ΚB信号通路 LEWIS肺癌 小鼠 

分 类 号：R285.5[医药卫生—中药学]