顶空气相色谱法测定藻酸双酯钠丙二醇酯基取代程度  

Determination of the propylene glycol ester group substitution degree of Propylene glycol alginate sodium sulfate by headspace gas chromatography

作　　者：陈欣桐 王悦[1,2] 宋玉娟 刘博[1,2] 范慧红 CHEN Xin-tong;WANG Yue;SONG Yu-juan;LIU Bo;FAN Hui-hong(Division of Biochemical drug,China Institute for Food and Drug Control,Beijing 102629,China;Key Laboratory of Chemical Drug Quality Research and Evaluation,Beijing 102629,China)

机构地区：[1]中国食品药品检定研究院生化药品室,北京102629 [2]化学药品质量研究与评价重点实验室,北京102629

出　　处：《中国海洋药物》2022年第3期43-49,共7页Chinese Journal of Marine Drugs

基　　金：海洋药物国家重点实验室开放性课题项目(LMDBKF201806)资助。

摘　　要：目的 建立藻酸双酯钠丙二醇酯基取代程度的气相测定方法。方法 利用强碱碱解藻酸双酯钠丙二醇酯基为1,2-丙二醇,按顶空气相色谱法进样分析。气相色谱柱为DB-WAX毛细管色谱柱(30 m×0.32 mm,0.5μm),载气为氮气,顶空平衡温度120℃,程序升温,氢火焰离子化检测器(FID)。结果 1,2-丙二醇质量浓度在0.1~3.0 mg/mL范围内线性关系良好(r=0.999 5),精密度RSD小于3%。结论 该方法灵敏度高、准确性好,有助于完善藻酸双酯钠质量标准研究,减少不良反应的发生。Objective To establish a method for the detection of 1,2-propanediol in alkaline hydrolysis solution to determine propylene glycol ester group substitution degree of Propylene glycol alginate sodium sulfate(PSS).Methods Hydrolyzed propylene glycol ester group of PSS to 1,2-propanediol with strong base, was determined by headspace gas chromatography. The column was DB-WAX(30 m × 0.32 mm, 0.5 μm);the carrier gas was nitrogen;headspace equilibrium temperature was 120 ℃, temperature programming method, hydrogen flame ionization detector(FID). Results The linearity of 1,2-propanediol was good within the range of 0.1~3.0 mg/mL(r = 0.999 5), and the precision RSD was less than 3%. Conclusion This method had high sensitivity and good accuracy, which was helpful to improve the quality standard research of PSS and reduce the occurrence of adverse reactions.

关 键 词：气相色谱法 顶空进样 藻酸双酯钠 丙二醇酯基取代程度 1 2-丙二醇 

分 类 号：R917[医药卫生—药物分析学]